Introduction: Diabetes mellitus, as a chronic metabolic disease, is associated with a wide range of kidney and liver disorders. The goal of this study was to indicate the effects of selenium nanoparticles on the function of kidney and liver enzymes in stereptozocin induced diabetic rats. Methods: In this study, 35 male wistar rats were divided into five groups (n=7): control, diabetic control and three experimental groups receiving selenium nanoparticle solutions at doses of 0.1, 0.2 and 0.4 mg/kg/BW respectively. To induce diabetes in rats, a single dose of streptozocin (60 mg/kg/BW) was injected intraperitoneally. After the experimental period, blood samples were collected from all groups and the blood factors associated with the liver enzymes and kidney factors were measured and analyzed. Results: The results indicated that the levels of fasting blood sugar in all groups treated with selenium nanoparticles had a significant reduction compared to the diabetic group (P≤0.05). Aspartate amino transferase had a significant reduction at the maximum dose compared to the diabetic group (P≤ 0.05). Also a significant decrease in the level of albumin was seen in the group treated with selenium nanoparticle (P≤ 0.05). Selenium nanoparticle treatment made a significant decreases in the levels of urea and ceratinine at the maximum dose (P≤ 0.05). Conclusion: The consumption of selenium nanoparticles in proper dosages may have beneficial effects on diabetic complications by lowering blood sugar as well as reducing the increased levels of the liver enzymes and kidney factors, albumin and cratinine resulting in the better function of kidney and liver.