%0 Journal Article %A Farrokhi, Majid Reza %A Emamghoreishi, Masoumeh %A Amiri, Atena %A Keshavarz, Mojtaba %T Neuroprotective effects of caffeine against beta-amyliod neurotoxicity: The involvement of glycogen synthase kinase-3β protein %J Physiology and Pharmacology %V 23 %N 3 %U http://ppj.phypha.ir/article-1-1447-en.html %R %D 2019 %K Caffeine, Amyloid-beta peptide, Glycogen synthase kinase, Neuroprotection., %X Introduction: The reduction of glycogen synthase kinase-3β protein level may correlate to the neuroprotective effects of antioxidant agents like caffeine. Therefore, we aimed to evaluate the impact of GSK-3β protein on neuroprotective effects of caffeine in the SHSY5Y cells exposed to beta-amyloid. Methods: We incubated SHSY5Ycells with beta-amyloid 25–35 and caffeine (0.6 and 1mM) for 24h. Cell viability was determined using MTT test. We used the western blotting technique to measure the glycogen synthase kinase-3β and phosphorylated glycogen synthase kinase-3β protein levels. Results: Caffeine (0.6 and 1mM) diminished beta-amyloid neurotoxicity and attenuated the beta-amyloid effects on the glycogen synthase kinase-3β protein level in a neuronal culture. Conclusion: Caffeine neuroprotective effects against beta-amyloid may correlate to glycogen synthase kinase-3β protein. %> http://ppj.phypha.ir/article-1-1447-en.pdf %P 150-153 %& 150 %! Caffeine neuroprotection via GSK-3β %9 Short communication %L A-10-1178-2 %+ Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran %G eng %@ 24765236 %[ 2019