Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1393
10
1
gregorian
2015
1
1
18
4
online
1
fulltext
en
اثر اسانس ترخون Artemisia dracunculus بر روی اسهال و زمان عبور رودهای مواد در دستگاه گوارش موش صحرایی
Effects of Artemisia dracunculus essential oil on diarrhea and intestinal transit time in rat gastrointestinal tract
Gastrointestinal Physiology/Pharmacology
Gastrointestinal Physiology/Pharmacology
Experimental research article
Experimental research article
Introduction: Artemisia dracunculus L. belongs to Asteraceae family, and is a medicinal plant widely used in
traditional medicine as a remedy for gastrointestinal disturbances. This study was undertaken to evaluate the effects of
essential oil of A. dracunculus (EOAD) on the rat alimentary tract.
Methods: The EOAD was extracted by Clevenger apparatus using hydrodistillation. LD50 was calculated based on
the Lorke’s method. The effects of EOAD (50–125 mg/kg) on intestinal transit time and diarrhea were investigated in
adult Wistar rats. EOAD was administered via oral route. For antidiarrheal effect evaluation, castor oil (2 mL/rat) was
administered intragastrically 30 min after EOAD (50-100 mg/kg) treatments and loperamide (3 mg/kg). The rat cages
were inspected hourly up to 4 hours for the presence of the characteristic diarrheal droppings, start time of diarrhea,
weight of stool, and the number of stool plates.
Results: The LD50 was 707.10 mg/kg. EOAD significantly inhibited intestinal motility at 125 mg/kg dose (P<0.05).
EO inhibitory effect was significantly (P<0.05) enhanced with simultaneous atropine. Castor oil caused diarrhea in all
animals in the control group in 93.83± 4.81 min. EOAD inhibited the castor oil-induced diarrhea at 75 and 100 mg/kg
doses. The EOAD delayed the onset of diarrhea, and produced a significant decrease in the frequency of defecation as
well as severity of diarrhea. It also protected the rats against diarrhea. In comparison with loperamid, the reference
antidiarrheal agent, the higher dose of EOAD demonstrated the same effective protection as castor oil-induced
diarrhoea.
Conclusion: These primary data indicated that the plant contains antidiarrheal constituents, which support the
popular therapeutic use of A. dracunculus for gastrointestinal disorders in traditional medicine.
Gastrointestinal disorders, A. dracunculus, Motility, Castor oil, Antidiarrhea
416
428
http://ppj.phypha.ir/browse.php?a_code=A-10-672-1&slc_lang=en&sid=1
Ghader
Jalilzadeh-Amin
قادر
جلیل زاده امین
g.jalilzadeh@urmia.ac.ir
3200319475328460014964
3200319475328460014964
Yes
Dept. of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Behzad
Mehrivar qarehdarvishlu
بهزاد
مهریور قره درویشلو
g.jalilzadeh@gmail.com
3200319475328460014965
3200319475328460014965
No
School of Veterinary Medicine, Urmia University, Urmia, Iran