en بررسی بیان ژن‮های 35P و 72P در تومورهای سرطان پستان القا شده توسط ان- نیتروزو ان- متیل یوریا (UMN) در موش صحرایی نژاد ویستار آلبینو Cell, Stem Cell and Cancer Cell, Stem Cell and Cancer Experimental research article Experimental research article <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt"><b style="line-height: 250%"><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">مقدمه: </span></b><span lang="FA" style="line-height: 250% font-size: 13pt font-family: 'B Mitra'">ان- نیتروزو ان- متیل یور</span><span lang="FA" style="font-family: 'B Mitra' line-height: 40px"><font size="3">یا</font></span><span lang="FA" style="line-height: 250% font-size: 12pt font-family: 'B Mitra'"><span style="color: rgb(0, 51, 204)"> </span>(</span><span dir="ltr" style="line-height: 250%"></span><span dir="ltr" style="line-height: 250% font-size: 12pt font-family: 'Times New Roman', serif"><span dir="ltr"></span>(NMU</span><span dir="rtl" style="line-height: 250%"></span><span style="line-height: 250% font-size: 13pt font-family: 'B Mitra'"><span dir="rtl"></span> </span><span lang="FA" style="line-height: 250% font-size: 13pt font-family: 'B Mitra'">از جمله سرطانزاهای شیمیایی از گروه نیتروزآمین هاست که در القای سرطان پستان در جوندگان (مانند رت) به کار می رود. این مدل سرطان پستان بسیار مشابه با سرطان پستان وابسته به استروژن در انسان می باشد. <span style="color: rgb(0, 0, 153)">به دنبال پژوهشهای قبلی ما و دیگران، در این مطالعه بیان دو ژن </span></span><span dir="ltr" style="line-height: 250% font-size: 12pt font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p53</span><span dir="rtl" style="line-height: 250%"></span><span style="line-height: 250% font-size: 13pt font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> </span><span lang="FA" style="line-height: 250% font-size: 13pt font-family: 'B Mitra' color: rgb(0, 0, 153)">و </span><span dir="ltr" style="line-height: 250% font-size: 12pt font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p27</span><span dir="rtl" style="line-height: 250%"></span><span lang="FA" style="line-height: 250% font-size: 13pt font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> در تومورهای سرطان پستان القا شده توسط </span><span dir="ltr" style="line-height: 250% font-size: 12pt font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">NMU</span><span dir="rtl" style="line-height: 250%"></span><span lang="FA" style="line-height: 250% font-size: 13pt font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> در رت بررسی شد.</span><span lang="FA" style="line-height: 250% font-size: 13pt font-family: 'B Mitra' color: rgb(0, 0, 153)"><o:p /></span></p> <p class="MsoNormal" dir="rtl" style="margin: 8pt 0cm 0.0001pt line-height: 250%"><b><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">روشها</span></b><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">: القای سرطان پستان در رت به واسطه 3 تزریق درون صفاقی </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">NMU</span><span dir="rtl"></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><span dir="rtl"></span> انجام شد؛ ولی به رت‌های گروه کنترل، سرم فیزیولوژی تزریق گردید. پس از ایجاد تومورها و رسیدن آنها به اندازه موردنیاز، رت‌های هر دو گروه فوق بیهوش، سپس تومورها و بافت‌های طبیعی پستان خارج شد و بیان دو ژن</span><span lang="FA" style="font-size: 12pt line-height: 250% font-family: 'B Mitra'"> </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">p53</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 250% font-family: 'B Mitra'"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">و </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">p27</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 250% font-family: 'B Mitra'"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">با روش </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">RT-PCR</span><span dir="rtl"></span><span style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><span dir="rtl"></span> <span lang="FA">در آنها بررسی گردید.</span></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><o:p /></span></p> <p class="MsoNormal" dir="rtl" style="margin: 8pt 0cm 0.0001pt line-height: 250%"><b><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">یافته ها</span></b><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">: تزریق </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">NMU</span><span dir="rtl"></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><span dir="rtl"></span> منجر به القای سرطان پستان در نمونه ها گردید. <span style="color: rgb(0, 0, 153)">نتایج </span></span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">RT-PCR</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)">حاکی از تغییر روبه افزایش بیان ژن </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p53</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)">(05/0</span><span dir="ltr" style="font-size: 13pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p> </span><span dir="rtl"></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span>) </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)">و تغییر رو به کاهش بیان ژن </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p27</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)">(05/0</span><span dir="ltr" style="font-size: 13pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p> </span><span dir="rtl"></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span>)</span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"> در تومورهای سرطان پستان القا شده در رت نسبت به بافت طبیعی پستان بود. این مطالعه برای اثبات تغییرات مشاهده شده با روش های دیگر ادامه دارد. ولی این تغییرات</span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"> با نتایج قبلی ما مبنی بر تغییرات بیان ژن‌های سیکلین </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">D1</span><span dir="rtl"></span><span style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">و </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif">p21</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif"><span dir="rtl"></span> </span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">هم‌خوانی دارد.</span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><o:p /></span></p> <p class="MsoNormal" dir="rtl" style="margin: 8pt 0cm 0.0001pt line-height: 250%"><b><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'">نتیجه گیری: </span></b><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)">مشاهدات این مطالعه بیانگر تغییرات متضاد بیان ژن‌های </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p53</span><span dir="rtl"></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> و </span><span dir="ltr" style="font-size: 12pt line-height: 250% font-family: 'Times New Roman', serif color: rgb(0, 0, 153)">p27</span><span dir="rtl"></span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra' color: rgb(0, 0, 153)"><span dir="rtl"></span> در تومورها نسبت به بافت سالم پستان بود.</span><span lang="FA" style="font-size: 13pt line-height: 250% font-family: 'B Mitra'"><o:p /></span></p> Introduction: N-nitroso-N-methylurea (NMU) is a carcinogen from nitrosamines family, which has been used to induce breast cancer in rodents. This model of breast cancer is very similar to the estrogen dependent breast cancer in human. As a continuation of our recent works, in the present study, the expressions of both p53 and p27 were investigated in NMU-induced breast cancer in Wistar Albino rats. Methods: Breast cancer was induced by intraperitoneal injection of NMU in Wistar Albino rats, while, normal saline was injected in the control group. After the tumors were formed and reached a proper size, the rats in both groups were sacrificed under anesthesia and samples were obtained from mammary tumors as well as normal breast tissue. Expressions of p53 and p27 genes were investigated by reverse-transcription polymerase chain reaction (RT- PCR). Results: NMU injection induced breast cancer in rats. RT-PCR data showed that p53 expression was increased (p>0.05), while p27 expression was decreased in rat mammary tumors compared with the normal tissue of normal rats (p>0.05). This study is being continued to confirm the results, but these findings are compatible with our previous data about cyclin D1 and p21 expressions in tumor tissue. Conclusion: The results indicate opposite changes in the expression of p53 and p27 in cancerous breast tissue in comparison with the normal breast tissue. سرطان پستان, ان نیتروزو ان متیل یوریا, p53, p27 Breast Cancer, N-Nitroso-N-Methylurea (NMU),p53, p27 337 346 http://ppj.phypha.ir/browse.php?a_code=A-10-40-1&slc_lang=en&sid=1 Mahshid Azizian مهشید عزیزیان azizian424@yahoo.com 3200319475328460014492 3200319475328460014492 No Dept. of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. S. Zahra Bathaie سیده زهرا بطحائی bahai_z@modares.ac.ir 3200319475328460014493 3200319475328460014493 Yes Dept. of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Mahboobe Ashrafi محبوبه اشرافی mashrafi@shirazu.ac.ir 3200319475328460014494 3200319475328460014494 No Dept. of Basic Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran. Reyhane Hoshyar ریحانه هوشیار hooshyar@bums.ac.ir 3200319475328460014495 3200319475328460014495 No Dept. of Biochemistry, Birjand University, Birjand, Iran