en Endocrine Physiology/Pharmacology Endocrine Physiology/Pharmacology Experimental research article Experimental research article <p dir="ltr" style="text-align: justify;"><span style="font-size: 12px;"><b>Introduction: </b><font face="Arial,Arial"><font face="Arial,Arial">Sandfish (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">Holothuria scabra</font></font></i><font face="Arial,Arial"><font face="Arial,Arial">) is a marine species generally sold as a raw material that has been dried even though the meat contains steroid hormone with high economic value, which has the potential to become a source of safe natural steroid hormone. This study was aimed to look at the potency of sandfish as an anti-aging for menopausal women. Sandfish could become a source of natural steroid for hormone replacement therapy to replace synthetic hormone that is proven to have negative impacts on health. </font></font></span><span style="font-size: 12px;"><b>Methods: </b><font face="Arial,Arial"><font face="Arial,Arial">This study used female rats (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">Rattus norvegicus</font></font></i><font face="Arial,Arial"><font face="Arial,Arial">) Sprague-Dawley Strain Variety II that were twelve weeks old and ovariectomized. In these animals, the bioassay test was conducted with the treatment of sandfish meat powder (SP) containing 30, 40, and 50 &mu;g steroid/100 g. At the end of the treatment, the examination was carried out toward the concentration of steroid in blood serum using radioimmunoassay (RIA) and the uterine weight. </font></font></span><span style="font-size: 12px;"><b>Results: </b><font face="Arial,Arial"><font face="Arial,Arial">The treatment with SP in 30 &mu;g steroid/100 g could increase the estradiol hormone in blood serum of test animals that were ovariectomized and produce the highest concentration of uterine weight. </font></font></span><span style="font-size: 12px;"><b>Conclusion: </b><font face="Arial,Arial"><font face="Arial,Arial">This study showed that a dose of SPcontaining 30 &mu;g steroid/100 g body weight had the highest potential as an anti-aging in menopausal women.</font></font></span></p> Anti-aging, Menopause, Sandfish, Steroid 206 214 http://ppj.phypha.ir/browse.php?a_code=A-10-921-1&slc_lang=en&sid=1 Etty Riani etty_riani_harsono@yahoo.com 3200319475328460016713 3200319475328460016713 Yes Department of Aquatic Resources Management, Faculty of Fishery and Marine Science, Bogor Agricultural University. Jl. Lingkar Akademik, Kampus IPB Darmaga, Bogor, West Java, Indonesia Chairunissa chnisa@yahoo.com 3200319475328460016714 3200319475328460016714 No Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis, Kampus IPB Darmaga, Bogor, West Java, Indonesia Hera Maheswari hera_maheshwari@yahoo.com 3200319475328460016715 3200319475328460016715 No Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis, Kampus IPB Darmaga, Bogor, West Java, Indonesia Muhammad Dzikrifishofa dzikrifishofa@gmail.com 3200319475328460016716 3200319475328460016716 No Regional General Hospital of Leuwiliang, Regency of Bogor, Cibeber I, Leuwiliang, Bogor, West Java, Indonesia Nastiti Kusumorini ntitik@yahoo.com 3200319475328460016717 3200319475328460016717 No Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis, Kampus IPB Darmaga, Bogor, West Java, Indonesia