Physiology and Pharmacology

fa High glucose condition down-regulates the inhibitory G-protein subunit, Gαi, in pheochromocytoma PC12 cells Cell, Stem Cell and Cancer Cell, Stem Cell and Cancer Experimental research article Experimental research article Introduction: G-proteins have an important role in the cell signaling of numerous receptors. The situation of G-proteins in health and disease and their critical role in the development of diabetic side effects is an interested scientific field. Here, the changes in the expression of G-protein subunits (Gαi, Gαs and Gβ) were evaluated in hyperglycemic situation of PC12 cells as a cellular model for the induction of diabetic side effect. Methods: Rat pheochromocytoma PC12 cells were grown in normal or high-glucose (4X normal glucose) medium. Cell viability was determined by MTT assay and the generation of intracellular reactive oxygen species (ROS) studied using fluorescence spectrophotometry. RT-PCR and immunobloting were performed to evaluate the expression of specific G-protein subunits in the levels of mRNA and protein, respectively. Results: In high glucose condition (100 mM glucose for 48h), the cell viability was significantly decreased and intracellular ROS increased. In addition, Gαi expression level was significantly decreased in hyperglycemic PC12 cells. However, the levels of Gαs and Gβ mRNAs and their proteins were not altered in high glucose-treated cells. Conclusion: The results demonstrate that deregulation or disruption in the signaling of Gai coupled receptors can be occurred in hyperglycemic condition. G-proteins, Gene expression, Hyperglycemia, PC12 cells 239 245 http://ppj.phypha.ir/browse.php?a_code=A-10-79-9&slc_lang=fa&sid=1 Saeed Esmaeili-Mahani semahani@yahoo.com 3200319475328460016896 3200319475328460016896 Yes Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran Zahra Hajializadeh zahra.hajalizadeh@gmail.com 3200319475328460016897 3200319475328460016897 No Laboratory of Molecular Neuroscience, Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran Shima Torkzadeh-Mahani shtmahani@yahoo.com 3200319475328460016898 3200319475328460016898 No Laboratory of Molecular Neuroscience, Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran