Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1395
8
1
gregorian
2016
11
1
20
4
online
1
fulltext
en
Melatonin and alpha lipoic acid as possible therapies for lopinavir/ritonavir-induced hepatotoxicity in albino rats
Pharmacokinetics/Dynamics
Pharmacokinetics/Dynamics
Experimental research article
Experimental research article
<p dir="ltr" style="text-align: justify;"><span style="font-size: 12px;"><b>Introduction: </b><font face="Arial,Arial"><font face="Arial,Arial">The use of lopinavir/ritonavir (LPV/r) has decreased morbidity and mortality due to human immunodeficiency virus (HIV); however its use could be impaired by hepatotoxicity. Therefore, this study was designed to investigate the effects of melatonin (MT) and alpha lipoic acid (ALA) on LPV/r-induced hepatotoxicity in male albino rats. </font></font></span><span style="font-size: 12px;"><b>Methods: </b><font face="Arial,Arial"><font face="Arial,Arial">Rats were divided into groups and treated with MT (10 mg/kg/day), ALA (10 mg/kg/day) and LPV/r (22.9/5.71, 45.6/11.4 and 91.2/22.9 mg/kg/day) for 60 days respectively. Rats were pretreated with MT (10 mg/kg), ALA (10 mg/kg) and combined doses of ALA and MT prior to treatment with LPV/r (22.9/5.71, 45.6/11.4 and 91.2/22.9 mg/kg/day) for 60 days. Rats were sacrificed and serum was collected and evaluated for liver enzymes. The liver was harvested and evaluated for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) levels. </font></font></span><span style="font-size: 12px;"><b>Results: </b><font face="Arial,Arial"><font face="Arial,Arial">Significant (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><0.05) decreases in baseline serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and liver MDA levels with increases in liver SOD, CAT and GSH levels were obtained in MT and ALA treated animals when compared to control. On the contrary, significant (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><0.05) and dose dependent increases in serum AST, ALT, ALP and liver MDA levels with decreases in liver SOD, CAT and GSH levels were obtained in LPV/r treated rats when compared to placebo control. However, LPV/r-induced changes in the above parameters were attenuated in MT and ALA pretreated rats. Attenuations were significantly (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><0.05) different in rats pretreated with combined doses of MT and ALA when compared to their individual doses. </font></font></span><span style="font-size: 12px;"><b>Conclusion: </b><font face="Arial,Arial"><font face="Arial,Arial">Results of this study showed that MT and ALA could be used for the treatment of LPV/r associated hepatotoxicity.</font></font></span></p>
Liver, Toxicity, Lopinavir/ritonavir, Antioxidants, Pretreatment, Rats
287
295
http://ppj.phypha.ir/browse.php?a_code=A-10-861-4&slc_lang=en&sid=1
Elias
Adikwu
adikwuelias@gmail.com
3200319475328460016923
3200319475328460016923
Yes
Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria
Brambaifa
Nelson
bamnel@gmail.com
3200319475328460016924
3200319475328460016924
No
Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria
Obianime
Wolfe Atuboyedia
3200319475328460016925
3200319475328460016925
No
Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria