Physiology and Pharmacology

en Effect of tempol, a synthetic antioxidant, on renal complications of L-NAME induced preeclampsia in rat Pharmacokinetics/Dynamics Pharmacokinetics/Dynamics Experimental research article Experimental research article Introduction: It has been suggested that oxidative stress has a crucial role in the pathophysiology of preeclampsia. In the present study, the effect of tempol, a synthetic antioxidant, on kidney injuries and oxidative stress was investigated in an experimental model of preeclampsia in rats. Methods: Preeclampsia was induced by oral administration of L-NAME to the rats on the day 10 of pregnancy. Animals were randomly divided into six groups (10-15 rats in each group); (I) normal pregnant (II) preeclamptic (III, IV, V) preeclamptic + tempol 20, 60 and 180 mg/kg/day, respectively, (VI) preeclamptic + hydralazine 10 mg/kg/day. Urine levels of sodium, potassium, creatinine, lactate dehydrogenase and 24 h protein, blood levels of creatinine and urea, in addition to malondialdehyde concentration in blood and kidney, as well as histological glomeruli changes were assessed. Results: L-NAME administration caused proteinuria and glomerular pathological changes. Tempol (20 and 60 mg/kg/day) significantly reduced plasma and renal (P<0.001) malondialdehyde levels and proteinuria (the biggest calculated P-value was less than 0.05) in preeclamptic rats. Tempol at the dose of 20 mg/kg/day improved the histological changes in preeclamptic animals, but the dose of 60 mg/kg/day restored histological findings. L-NAME did not change the other measured parameters. Hydralazine and highest dose of tempol (180 mg/kg/day) failed to affect biochemical and histological changes in experimental preeclampsia. Conclusion: Renal complications of experimental preeclampsia such as proteinuria and glomerular injuries can be prevented by tempol. The desired effects of tempol depend on its dose. Tempol, Rats, Preeclampsia, Oxidative Stress, Proteinuria. 241 250 http://ppj.phypha.ir/browse.php?a_code=A-10-984-1&slc_lang=en&sid=1 Mohammad Sharif Talebianpoor m.talebianpoor @yums.ac.ir 3200319475328460018193 3200319475328460018193 No Herbal Medicine Research Center, School of Medicine, Yasouj University of Medical Sciences, Yasouj, Iran Seyed Mohammad Owji smowji@sums.ac.ir 3200319475328460018194 3200319475328460018194 No Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran Mohsen Goharinia gohariniam@sums.ac.ir 3200319475328460018195 3200319475328460018195 No Department of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran Hossein Mirkhani mirkhan@sums.ac.ir 3200319475328460018196 3200319475328460018196 Yes Department of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran