en Gastrointestinal Physiology/Pharmacology Gastrointestinal Physiology/Pharmacology Experimental research article Experimental research article <span style="font-size: 12px;"><p align="LEFT"></p></span> <p style="text-align: justify;"><span style="font-size: 12px;"><span style="color: rgb(0, 0, 0);"><b>Introduction: </b><font face="Arial,Arial"><font face="Arial,Arial">The incidence rate of gastric erosions and ulcers in diabetic patients are higher due to failure of mucosal antioxidant defense and maintain enough blood flow. The present study evaluated the gastro-protective effect of sodium hydrosulfide (NaHS) against indomethacin-induced gastric lesions in diabetic rats. <span style="color: rgb(0, 0, 0);"><b>Methods: </b><font face="Arial,Arial"><font face="Arial,Arial">In order to test anti-ulcer activity of NaHS against indomethacin, four diabetic groups of rats including diabetic control and 3 NaHS-treated groups received a single dose of physiologic saline or NaHS at 320, 640 and 1280 &mu;g/kg respectively, 30 min before ulcer induction by indomethacin. Five hours later, the animals were killed and their stomachs were removed for macroscopically and microscopically evaluations. In order to evaluate the antacid effect of NaHS, 4 groups of diabetic rats received physiologic saline or NaHS at 320, 640 and 1280 &mu;g/kg and 30 min later anesthetized, underwent a midline laparotomy and then their pylorus ligated. Five hours later, the animals were killed, their stomachs were removed and pH of gastric effluents were measured. <span style="color: rgb(0, 0, 0);"><b>Results: </b><font face="Arial,Arial"><font face="Arial,Arial">Indomethacin induced gastric lesions in glandular part of the stomach. NaHS at 640 and 1280 &mu;g/kg significantly decreased the indomethacin-induced gastric lesions in diabetic rats. The pH of gastric effluents and mucus content increased by NaHS at doses of 640 and 1280 &mu;g/kg. Macroscopic and microscopic observations showed that mucosal erosions induced by indomethacin were significantly inhibited by NaHS. <span style="color: rgb(0, 0, 0);"><b>Conclusion: </b><font face="Arial,Arial"><font face="Arial,Arial">results suggest NaHS through decreasing the rate of gastric acid output and increasing the mucus production, protected the gastric mucosa against indomethacin-induced gastric lesions in diabetic rats. </font></font></span></font></font></span></font></font></span></font></font></span></span></p> Diabetes, Gastric acid secretion, sodium hydrosulfide, Rat 48 53 http://ppj.phypha.ir/browse.php?a_code=A-10-35-6&slc_lang=en&sid=1 Nasim Nazariani nazariani64@yahoo.com 3200319475328460019148 3200319475328460019148 No Biology Department, Payame Noor University, Tehran, Iran Seyyed Ali Mard alimard77@gmail.com; mard-sa@ajums.ac.ir 3200319475328460019149 3200319475328460019149 Yes Alimentary Research Center, Physiology Research Center (PRC), Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Sima Nasri s_nasri2000@yahoo.com 3200319475328460019150 3200319475328460019150 No Biology Department, Payame Noor University, Tehran, Iran Ali Veisi 3200319475328460019151 3200319475328460019151 No Behbahan Faculty of Medical Sciences, Behbahan, Iran