en Renal Physiology/Pharmacology Renal Physiology/Pharmacology Experimental research article Experimental research article <span style="font-size: 12px;"><p align="LEFT"></p></span> <p style="text-align: justify;"><span style="font-size: 12px;"><span style="color: rgb(0, 0, 0);"><b>Introduction: </b><font face="Arial,Arial"><font face="Arial,Arial">Renal ischemia-reperfusion (RIR) may disturb renin-angiotensin system components. In this study, the effects of Mas receptor (A779) and AT</font></font><font face="Arial,Arial"><font face="Arial,Arial">2 </font></font><font face="Arial,Arial"><font face="Arial,Arial">receptor (PD123319) antagonists were examined in RIR rats. <span style="color: rgb(0, 0, 0);"><b>Methods: </b><font face="Arial,Arial"><font face="Arial,Arial">Total 60 male and female Wistar rats were assigned into 10 groups (n=6 in each group), including sham-operated group, RIR groups treated with the vehicle, A779, PD123319, or A779+PD123319. The rats were subjected to 30 minutes renal ischemia followed by 75 minutes reperfusion and the vehicle/antagonists were started to infuse 15 minutes after beginning of reperfusion for 60 min. Mean arterial pressure (MAP) and renal perfusion pressure responses to antagonists were assessed. Measurements for kidney function parameters also were performed. All the measurements were made at the end of 60 min vehicle/antagonist infusion. <span style="color: rgb(0, 0, 0);"><b>Results: </b><font face="Arial,Arial"><font face="Arial,Arial">MAP has altered significantly during RIR times (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial">=0.004), but no significant difference was observed between two genders. The RIR itself in injured rats (compared to sham operated rats) decreased urine flow (UF), creatinine clearance (Ccr), filtrate load of sodium (FNa) and sodium excretion rate (ENa) significantly in both genders (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><0.05). The antagonists infusion caused significant decrease in Ccr and FNa in male and female rats subjected to RIR when compared with vehicle (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><0.05), but the UF decreased significantly (</font></font><i><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><0.05) only in PD123319 treated groups; however, there was no significant difference in ENa between the RIR groups in both genders. <span style="color: rgb(0, 0, 0);"><b>Conclusion: </b><font face="Arial,Arial"><font face="Arial,Arial">Our findings showed the importance role of Mas receptor and AT</font></font><font face="Arial,Arial"><font face="Arial,Arial">2 </font></font><font face="Arial,Arial"><font face="Arial,Arial">receptor on renal function after kidney ischemia/reperfusion in RIR rat model. </font></font></span></font></font></span></font></font></span></font></font></span></span></p> Renal ischemia-reperfusion, Mas receptor, AT2 receptor, Renal Function, Gender 133 140 http://ppj.phypha.ir/browse.php?a_code=A-10-788-5&slc_lang=en&sid=1 Alireza Samimiat alireza_samimiat@yahoo.com 3200319475328460019417 3200319475328460019417 No Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Mohammad Sedigh Khosravi ms_khosravi1995@yahoo.com 3200319475328460019418 3200319475328460019418 No Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Jalal Hassanshahi hasanshahij@gmail.com 3200319475328460019419 3200319475328460019419 No Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Mehdi Nematbakhsh nematbakhsh@med.mui.ac.ir 3200319475328460019416 3200319475328460019416 Yes Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran