en Others Others Experimental research article Experimental research article <div style="text-align: justify;">Introduction: Candidiasis represents a major threat to the life and health in immune-compromised individuals. The number of antifungal drugs is limited for the treatment of candidiasis. Combination therapy is one of the most frequently used techniques to alleviate this problem. Methods: Clinical isolates of Candida albicans were obtained from the immune-compromised patients. Antifungal susceptibilities to fluconazole and amphotericin B alone and in combination were performed by broth microdilution method. Eventually direct microscopic observation, time-kill kinetic assay, biomass and metabolic activity of the hypha, Sap enzyme activity and expression of SAP3 gene were carried out in C. albicans. Results: Combination of fluconazole with amphotericin B demonstrated synergistic and partial synergistic effects with fractional inhibitory concentration index ranged from 0.031 to 0.75. The data indicated that combination of fluconazole with amphotericin B exerted antifungal effects through reducing time-kill kinetic, yeast&ndash; hyphal transition, biomass and metabolic activity of the hypha and Sap enzyme activity in C. albicans. Additionally, the expression levels of the SAP3 gene were significantly down regulated (P<0.001) in C. albicans treated with combination of fluconazole with amphotericin B. Conclusion: Taken together, these events may confirm the potential uses of combination of fluconazole with amphotericin B against C. albicans. The results suggest that SAP3 gene could be probable target of synergistic interaction of fluconazole and amphotericin B in C. albicans.</div> Amphotericin B, Candida albicans, Fluconazole, SAP3 195 204 http://ppj.phypha.ir/browse.php?a_code=A-10-1068-1&slc_lang=en&sid=1 Alireza Khodavandi khodavandi@iaug.ac.ir 3200319475328460020038 3200319475328460020038 No Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran Fahimeh Alizadeh f.alizadeh@iauyasooj.ac.ir 3200319475328460020039 3200319475328460020039 Yes Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran Fatemeh Khezrian f.Khezrian@iauyasooj.ac.ir 3200319475328460020040 3200319475328460020040 No Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran