Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1397
3
1
gregorian
2018
6
1
22
2
online
1
fulltext
en
Evaluation of the GABAA receptor on pain sensitivity in male rat pretreated with Valeriana officinalis extract using formalin test
Pain and addiction
Neurophysiology/Pharmacology
Experimental research article
Experimental research article
<span style="font-size: 12px;"><p align="LEFT"></p></span>
<p style="text-align: justify;"><span style="font-size: 12px;"><span style="color: rgb(0, 0, 0);"><b>Introduction: : </b><font face="Arial,Arial"><font face="Arial,Arial">It seems that </font></font><i><font face="Arial,Arial"><font face="Arial,Arial">Valeriana officinalis </font></font></i><font face="Arial,Arial"><font face="Arial,Arial">(valerian) extract through gamma-amino-butyric acid A (GABA</font></font><font face="Arial,Arial"><font face="Arial,Arial">A</font></font><font face="Arial,Arial"><font face="Arial,Arial">) receptor possesses analgesic effect. The aim of the present study was to investigate the effect of muscimol and picrotoxin on pain sensitivity in male rats pretreated with valerian extract using the formalin test. <span style="color: rgb(0, 0, 0);"><b>Methods: </b><font face="Arial,Arial"><font face="Arial,Arial">Thirty-five male rats weighing 200-250g in standard temperature 20±2 °C and light cycle of 12/12h used. Animals were randomly divided to 7 groups: sham 1 (injection of saline); sham 2 (pretreated with valerian + ICV injection of artificial cerebro spinal fluid); experimental1 (injection of valerian extract); experimental 2 or 3 (pretreated with valerian extract + ICV injection of muscimol 250 and 500 ng/rat); experimental 4 or 5 (pretreated with valerian extract + ICV injection of picrotoxin 250 and 500 ng/rat). Valerian extract 400 mg/kg was administrated by intraperitoneal injection. Pain evaluation was done by the formalin test. Lateral ventricles cannulated unilaterally by the stereotaxic procedure. <span style="color: rgb(0, 0, 0);"><b>Results: </b><font face="Arial,Arial"><font face="Arial,Arial">Data showed that valerian extract significantly decreased pain sensitivity in the late phase of the formalin test in comparison to sham 1 group. Muscimol in both doses significantly decreased pain in comparison to sham 1, while at the dose of 500 ng/rat significantly increased pain sensitivity in comparison to sham 2 at late phases of formalin test. Picrotoxin at both doses significantly decreased pain sensitivity in comparison to sham 1, while significantly increased pain sensitivity in comparison to the sham 2 at late phases of formalin test. <span style="color: rgb(0, 0, 0);"><b>Conclusion: </b><font face="Arial,Arial"><font face="Arial,Arial">According to present results, valerian extract had analgesic effect through the GABA</font></font><font face="Arial,Arial"><font face="Arial,Arial">A </font></font><font face="Arial,Arial"><font face="Arial,Arial">receptor. </font></font></span></font></font></span></font></font></span></font></font></span></span></p>
Valeriana officinalie, Musciol, Picrotoxin, Pain, Rat
118
123
http://ppj.phypha.ir/browse.php?a_code=A-10-249-8&slc_lang=en&sid=1
Mahnaz
Taherianfard
taherian@shirazu.ac.ir
3200319475328460019411
3200319475328460019411
Yes
Physiology Division of Basic Science Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mehdi
Karamifard
karamifard@yahoo.com
3200319475328460019412
3200319475328460019412
No
Physiology Division of Basic Science Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran