en Nervous system (others) Others Experimental research article Experimental research article <span style="font-size: 12px;"><p align="LEFT"></p></span> <p style="text-align: justify;"><span style="font-size: 12px;"><span style="color: rgb(0, 0, 0);"><b>Introduction: </b><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">A specific role of </font></font></font><font face="Arial,Arial"><font face="Arial,Arial">dopamine D2 receptor </font></font><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">signaling of midbrain and hypothalamic dopaminergic systems has not been yet identified in energy homeostasis. Here, we investigated effects of intra-ventromedial hypothalamus (VMH) administration of the D2 receptor agonist (quinpirole) and antagonist (sulpiride) on plasma leptin and glucose levels in fasted rats. <span style="color: rgb(0, 0, 0);"><b>Methods: </b><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">A guide cannula was stereotaxically implanted in the VMH of male Wistar rats (n=6/group). In experiment day, the fasted rats (20-24h) received </font></font></font><font face="Arial,Arial"><font face="Arial,Arial">a recommended </font></font><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">dose of D2 receptor agonist (quinpilroe: 0.5&mu;g), antagonist (sulpiride: 0.005&mu;g) and saline (0.5&mu;l) injected into the VMH. Blood samples were collected at 0, 30 and 60 min after injection, and plasma leptin and glucose were measured by Eliza kit and glucose oxidase method, respectively. <span style="color: rgb(0, 0, 0);"><b>Results: </b><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">Plasma leptin significantly increased in a time dependent manner in quinpirole group compared to control (</font></font></font><i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial"><0.01), while sulpiride markedly suppressed it (</font></font></font><i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial"><0.001). Increase in glucose levels was time dependently robust in quinpirole group (</font></font></font><i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial"><0.00). A significant reduction was observed in glucose levels in sulpiride compared to control group (</font></font></font><i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">P</font></font></font></i><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial"><0.05). There was also a negative correlation between glucose and leptin plasma levels in drug-treated rats. <span style="color: rgb(0, 0, 0);"><b>Conclusion: </b><font face="Arial,Arial"><font face="Arial,Arial"><font face="Arial,Arial">These data suggest that altered the VMH D2-mediated neurotransmission might contribute to an alteration in the metabolic phenotype (leptin secretion and plasma glucose level) of rats. </font></font></font></span></font></font></font></span></font></font></font></span></font></font></font></span></span></p> Dopamine, D2 receptor, Glucose, leptin, Ventromedial nucleus 124 132 http://ppj.phypha.ir/browse.php?a_code=A-10-766-3&slc_lang=en&sid=1 Maedeh Ghasemi ghasealaei@med.mui.ac.irmi.m.ph@gmail.com 3200319475328460019502 3200319475328460019502 No Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Nasrin Mehranfard nasrin_mehranfar@yahoo.com 3200319475328460019503 3200319475328460019503 No Neurophysiolog Research Center, Urmia University of Medical Sciences, Urmia, Iran HojjatAllah Alaei alaei@med.mui.ac.ir 3200319475328460019504 3200319475328460019504 Yes Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran