Physiology and Pharmacology

en High and low temperatures affect rat hippocampal synaptosome’s viability and functions Nervous system (others) Others Experimental research article Experimental research article Introduction: Synaptosomes are sealed particles that contain mitochondria, cytoskeleton and vesicles which are necessary to synaptic events like neurotransmitter release and uptake in the nervous system. However, the effect of high and low temperatures on synaptosome membrane integrity and function during a time course after its extraction is less known. The purpose of this study was to assess synaptosome viability and function at 37, 4°C and room temperature (RT) during 6 hours after its extraction. Methods: Hippocampi of 40 male Wistar rats were used for synaptosome preparation. To ensure synaptosome membrane integrity and function, lactate dehydrogenase activity (LDH) and GABA uptake were assessed during 6 successive hours after their extraction at 37, 4°C and RT. Results: Our results showed that at 37°C, synaptosome membrane integrity was reduced 3 hours but at 4°C and RT, it occurred 5 hours following their extraction. The results of synaptosome function analysis coincide with LDH enzyme assay data, meaning that GABA uptake faced a 50% reduction from the initial value at 37°C after 3 hours and at RT after 5 hours. We also found that GABA uptake was reduced at 4°C in the first hour after extraction because the low temperature inhibits GABA transporters. Conclusion: Synaptosomes preserved their viability and function at RT, 37 and 4°C at least for 3 hours after extraction and reduced over time. For long term application of synaptosomes, it is better to keep them at 4°C. Synaptosome, Viability, LDH activity, GABA uptake, Hippocampus 73 81 http://ppj.phypha.ir/browse.php?a_code=A-10-448-3&slc_lang=en&sid=1 Sahand Asharfpour sah.ashrafpour@gmail.com 3200319475328460019384 3200319475328460019384 No Student Research Committee, Babol University of Medical Sciences, Babol, Iran Fereshteh Pourabdolhossein F.pourabdolhossein@mubabol.ac.ir 3200319475328460019385 3200319475328460019385 Yes Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Forough Ebrahim Tabar forouq_ebr_95@yahoo.com 3200319475328460019386 3200319475328460019386 No Student Research Committee, Babol University of Medical Sciences, Babol, Iran Manouchehr Ashrafpour mnrashrafpour@yahoo.com 3200319475328460019388 3200319475328460019388 No Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Mojdeh Navidhamidi navidhamidi@gmail.com 3200319475328460019389 3200319475328460019389 No Schools of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran Sima Shahabi sima.shahabi@ymail.com 3200319475328460019387 3200319475328460019387 No Physiology Departments, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran Maryam Ghasemi-Kasman maryam.ghasemi65@gmail.com 3200319475328460019390 3200319475328460019390 No Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Alireza Asgari arasgarik@yahoo.com 3200319475328460019391 3200319475328460019391 No Aerospace Medicine Research Center, AJA Medical Sciences University, Tehran, Iran