en Neurophysiology/Pharmacology Neurophysiology/Pharmacology Short communication Short communication <div style="text-align: justify;"><strong>Introduction:</strong> The reduction of glycogen synthase kinase-3&beta; protein level may correlate to the neuroprotective effects of antioxidant agents like caffeine. Therefore, we aimed to evaluate the impact of GSK-3&beta; protein on neuroprotective effects of caffeine in the SHSY5Y cells exposed to beta-amyloid. <strong>Methods:</strong> We incubated SHSY5Ycells with beta-amyloid 25&ndash;35 and caffeine (0.6 and 1mM) for 24h. Cell viability was determined using MTT test. We used the western blotting technique to measure the glycogen synthase kinase-3&beta; and phosphorylated glycogen synthase kinase-3&beta; protein levels.<strong> Results:</strong> Caffeine (0.6 and 1mM) diminished beta-amyloid neurotoxicity and attenuated the beta-amyloid effects on the glycogen synthase kinase-3&beta; protein level in a neuronal culture. <strong>Conclusion: </strong>Caffeine neuroprotective effects against beta-amyloid may correlate to glycogen synthase kinase-3&beta; protein.</div> Caffeine, Amyloid-beta peptide, Glycogen synthase kinase, Neuroprotection. 150 153 http://ppj.phypha.ir/browse.php?a_code=A-10-1178-2&slc_lang=en&sid=1 Majid Reza Farrokhi farokhim@sums.ac.ir 3200319475328460029589 3200319475328460029589 No Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Masoumeh Emamghoreishi emamm@sums.ac.ir 3200319475328460029590 3200319475328460029590 No Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Atena Amiri neuroscien@sums.ac.ir 3200319475328460029591 3200319475328460029591 No Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Mojtaba Keshavarz mkeshavar@sums.ac.ir 3200319475328460029592 3200319475328460029592 Yes Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran