Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1398
7
1
gregorian
2019
10
1
23
3
online
1
fulltext
en
Rosmarinic acid-rich extract from Ocimum basilicum L. decreases hyperlipidemia in high fat diet-induced hyperlipidemic mice and prevents plasma lipid oxidation
Pharmacokinetics/Dynamics
Pharmacokinetics/Dynamics
Experimental research article
Experimental research article
<div style="text-align: justify;"><strong>Introduction:</strong> In this study, we investigated the effect of basil rosmarinic acid-rich extract on mice lipid metabolism, low-density lipoprotein oxidation and antiradical property. <strong>Methods:</strong> The rosmarinic acid rich-extract was used to treat male mice. Mice were divided into four groups of seven mice and treatment was performed daily and orally for 9 weeks. The antihyperlipidemic effect was evidenced by the measurement of plasma and liver lipid profiles. Thiobarbituric acid reactive substances assay was used to measure the antioxidant capacity of the phenolic extract using mice plasma rich in low density lipoprotein (LDL). The antioxidant activity of the extract was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity and the measurement of oxidative β-carotene bleaching. <strong>Results:</strong> The extract exerts a significant decrease in plasma total cholesterol, triglycerides and LDL-cholesterol. Similar results were observed in liver total cholesterol and triglycerides. The phenolic extract prevents lipoprotein oxidation by 93% at a dose of 25μg/ml. We also note that the extract scavenges DPPH radical in a dose-dependent manner with an IC50= 12.45±0.18 μg/ml. Besides, the extract inhibits the oxidation process of β-carotene, the IC50 was 12.16±0.19 μg/ml. HPLC analysis shows that the extract contains caftaric acid (2.9%), caffeic acid (4.3%), chicoric acid (5.5%) and rosmarinic acid (87.3%) which is the major compound. <strong>Conclusion:</strong> The results obtained suggest that the extract may be an important source of natural compounds that can be exploited as a substrate to develop new treatment of hyperlipidemia and atherosclerosis.</div>
Ocimum basilicum L., Rosmarinic acid, Lipid metabolism, Lipoprotein oxidation, Free radical scavenging.
197
207
http://ppj.phypha.ir/browse.php?a_code=A-10-1196-1&slc_lang=en&sid=1
Ilham
Touiss
i.touiss@ump.ac.ma
3200319475328460029615
3200319475328460029615
Yes
Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, University Mohamed I, Oujda, Morocco
Mohamed
Harnafi
3200319475328460029616
3200319475328460029616
No
Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, University Mohamed I, Oujda, Morocco
Saloua
Khatib
3200319475328460029617
3200319475328460029617
No
Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, University Mohamed I, Oujda, Morocco
Oussama
Bekkouch
3200319475328460029618
3200319475328460029618
No
Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, University Mohamed I, Oujda, Morocco
Khadija
Ouguerram
3200319475328460029619
3200319475328460029619
No
Thorax Institute, UMR-S 1087/UMR-C 6291, Nantes, France
Souliman
Amrani
3200319475328460029620
3200319475328460029620
No
Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, University Mohamed I, Oujda, Morocco
Hicham
Harnafi
3200319475328460029621
3200319475328460029621
No
Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, University Mohamed I, Oujda, Morocco