en Pharmacokinetics/Dynamics Pharmacokinetics/Dynamics Experimental research article Experimental research article <div style="text-align: justify;"><strong>Introduction: </strong>Pregabalin (PGB) is an analog of gamma-aminobutyric acid (GABA) with antinociceptive, antihyperalgesic and antiallodynic properties which frequently used in clinical pain management. Effect of PBG in neuropathic pain, incisional-inflammatory injury, post-operational pain, chronic pain and experimental pain models have already shown. It has been already known that muscarinic and serotonergic-2A receptors have a role in pain transmission. <strong>Methods:</strong> in this study, role of muscarinic and serotonergic-2A receptors in antinociceptive effect of pregabalin were evaluated with hot-plate and tail flick tests and effects of administered drugs on locomotor activity were measured with automated activity cage. <strong>Results:</strong> PGB treatment (30 and 100mg/kg) caused longer latency in hot plate and tail flick tests than saline group. That antinociceptive effect of pregabalin abolished by ketanserin (1mg/kg) and atropine (1mg/kg) treatment. <strong>Conclusion:</strong> However, there is lack of knowledge about role of nociceptive pathways underlying pregabalin mediated antinociception. Our results suggest that cholinergic and serotonergic systems have a role in antinociceptive effect of PGB which has seen in these somatic pain tests.</div> Pregabalin, Somatic pain, Ketanserin, Atropine. 302 308 http://ppj.phypha.ir/browse.php?a_code=A-10-1207-1&slc_lang=en&sid=1 Mohamad Hallak 3200319475328460029663 3200319475328460029663 No Erciyes University, School of Medicine, Deparment of Pharmacology 38039 Melikgazi, Kayserı, Turkey Hakan Balci 3200319475328460029664 3200319475328460029664 No Ondokuz Mayıs University, School of Medicine, Deparment of Pharmacology 55139, Atakum, Samsun, Turkey Caner Günaydın 3200319475328460029665 3200319475328460029665 No Ondokuz Mayıs University, School of Medicine, Deparment of Pharmacology 55139, Atakum, Samsun, Turkey S. Sirri Bilge ssbilge@omu.edu.tr 3200319475328460029666 3200319475328460029666 Yes Ondokuz Mayıs University, School of Medicine, Deparment of Pharmacology 55139, Atakum, Samsun, Turkey