Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1398
7
1
gregorian
2019
10
1
23
3
online
1
fulltext
en
Quercetin enhanced proliferation of neural stem/ progenitor cells through Nrf2-proteasome pathway
Cell, Stem Cell and Cancer
Cell, Stem Cell and Cancer
Experimental research article
Experimental research article
<div style="text-align: justify;"><strong>Introduction:</strong> Quercetin, a natural flavonoid, has been suggested as a stimulant of endogenous neural stem cell proliferation; but, its underlying mechanism is unclear. Considering that Nrf2 and proteasome pathways have important role in cell proliferation, the objective of this study was to evaluate the effects of different concentrations of quercetin on Nrf2 protein levels and proteasomal activity in neural stem/progenitor cells (NS/PCs) in relation to NS/PCs viability and proliferation. <strong>Methods:</strong> NS/PCs of rat fetal ganglionic eminence were cultured and exposed to different concentrations of quercetin (1, 5 and 15μM) or DMSO for 7 days. The size and number of neurospheres and percentage of BrdU positive cells were measured as indexes of cell proliferation. MTT assay was used for evaluating the cell viability. Proteasomal activity and Nrf2 protein levels were determined using proteasomal activity kit and western immunoblotting, respectively. <strong>Results:</strong> Quercetin increased the percentage of BrdU positive cells, size and number of neurospheres and viability of NS/PCs and increased Nrf2 protein levels and 20S proteasome activity in comparison to DMSO. The effects of quercetin on NS/PCs proliferation, Nrf2 levels and proteasome activity were concentration-dependent. <strong>Conclusion:</strong> The results of this study indicated that quercetin increased Nrf2 levels and proteasome activity in parallel to enhanced NS/PCs proliferation in a concentration-dependent manner. These findings suggest that quercetin may exert its stimulatory effect on NS/PCs proliferation through the enhancement of Nrf2-proteasome pathway. Quercetin as activators of Nrf2 and proteasome can be suggested as a potential treatment for neurodegenerative conditions to promote endogenous cell proliferation.</div>
Quercetin, Nrf2, Proteasome, Neural stem cells, Proliferation.
154
165
http://ppj.phypha.ir/browse.php?a_code=A-10-204-5&slc_lang=en&sid=1
Parisa
Sarkoohi
3200319475328460029593
3200319475328460029593
No
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Hadi
Aligholi
3200319475328460029594
3200319475328460029594
No
Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
Atena
Amiri
3200319475328460029595
3200319475328460029595
No
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Marzieh
Mahdavipour
3200319475328460029596
3200319475328460029596
No
Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran
Zahra
Zeraatpisheh
3200319475328460029597
3200319475328460029597
No
Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
Masoumeh
Emamghoreishi
emamm@sums.ac.ir
3200319475328460029598
3200319475328460029598
Yes
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran