Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1398
12
1
gregorian
2020
3
1
24
1
online
1
fulltext
en
Sub-threshold electrical stimulation improves wingless-type3 and eukaryotic initiation factor-2α expression in the presence of exogenous astrocyte in the rat model of spinal cord injury
Neurophysiology/Pharmacology
Neurophysiology/Pharmacology
Experimental research article
Experimental research article
<div style="text-align: justify;"><strong>Introduction:</strong> Spinal cord injury (SCI) is a condition which can lead to permanent loss of neurons, glial and precursor cells. According to the positive influences of electrical stimulation in the neurogenesis, we hypothesized that sub-threshold electrical stimulation in the presence of exogenous astrocyte may trigger the differential regulation of wingless-type3 (Wnt-3) and eukaryotic initiation factor-2α (eIF2α) mediators in spinal cord injured rats. <strong>Methods:</strong> Forty male Wistar rats (weighing 250-280g) were randomly divided into four groups: sham, SCI, SCI+astrocyte and SCI+astrocyte which followed by electrical stimulation. We evaluated the glial fibrillary acidic protein (GFAP), doublecortin, Wnt-3 and eIF2α proteins by immunofluorescence and immunoblotting techniques. <strong>Results:</strong> The results show that expression of Wnt-3 and eIF2α proteins significantly enhanced after 14 days in the electrical stimulation+ SCI+astrocyte group in comparison with SCI and SCI+astrocyte groups. Also, the expression of GFAP cells was significantly increased after 14 days by electrical stimulation compared with other groups. Electrical stimulation had no effect on expression of doublecortin after 14 days. <strong>Conclusion:</strong> This survey demonstrates that sub-threshold electrical stimulation up-regulates Wnt-3 and eIF2α mediators. Also, GFAP marker expression has been increased in animals subjected to electrical stimulation. But there are no evidences based on doublecortin expression as a neurogenesis biomarker.</div>
Spinal cord injury, Electrical stimulation, GFAP, Doublecortin, Wingless-type3.
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45
http://ppj.phypha.ir/browse.php?a_code=A-10-180-2&slc_lang=en&sid=1
Meysam
Ghorbani
3200319475328460029372
3200319475328460029372
No
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Parviz
Shahabi
shahabip@tbzmed.ac.ir
3200319475328460029373
3200319475328460029373
Yes
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Pouran
Karimi
pouran.karimi@yahoo.com
3200319475328460029374
3200319475328460029374
No
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad
Javan
3200319475328460029375
3200319475328460029375
No
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Soheila
Bani
3200319475328460029376
3200319475328460029376
No
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Saba
Hoseini
3200319475328460029377
3200319475328460029377
No
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
Hamid
Soltani-Zangbar
3200319475328460029378
3200319475328460029378
No
Neuroscience Research Center, Tabriz University of Medical Sciences, Faculty of Advanced Medical Sciences, Tabriz, Iran
Behnaz
Sadeghzadeh-Oskouei
3200319475328460029379
3200319475328460029379
No
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran