en Neurophysiology/Pharmacology Neurophysiology/Pharmacology Experimental research article Experimental research article <div style="text-align: justify;"><strong>Introduction:</strong> Spinal cord injury (SCI) is a condition which can lead to permanent loss of neurons, glial and precursor cells. According to the positive influences of electrical stimulation in the neurogenesis, we hypothesized that sub-threshold electrical stimulation in the presence of exogenous astrocyte may trigger the differential regulation of wingless-type3 (Wnt-3) and eukaryotic initiation factor-2&alpha; (eIF2&alpha;) mediators in spinal cord injured rats. <strong>Methods:</strong> Forty male Wistar rats (weighing 250-280g) were randomly divided into four groups: sham, SCI, SCI+astrocyte and SCI+astrocyte which followed by electrical stimulation. We evaluated the glial fibrillary acidic protein (GFAP), doublecortin, Wnt-3 and eIF2&alpha; proteins by immunofluorescence and immunoblotting techniques. <strong>Results:</strong> The results show that expression of Wnt-3 and eIF2&alpha; proteins significantly enhanced after 14 days in the electrical stimulation+ SCI+astrocyte group in comparison with SCI and SCI+astrocyte groups. Also, the expression of GFAP cells was significantly increased after 14 days by electrical stimulation compared with other groups. Electrical stimulation had no effect on expression of doublecortin after 14 days. <strong>Conclusion:</strong> This survey demonstrates that sub-threshold electrical stimulation up-regulates Wnt-3 and eIF2&alpha; mediators. Also, GFAP marker expression has been increased in animals subjected to electrical stimulation. But there are no evidences based on doublecortin expression as a neurogenesis biomarker.</div> Spinal cord injury, Electrical stimulation, GFAP, Doublecortin, Wingless-type3. 34 45 http://ppj.phypha.ir/browse.php?a_code=A-10-180-2&slc_lang=en&sid=1 Meysam Ghorbani 3200319475328460029372 3200319475328460029372 No Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Parviz Shahabi shahabip@tbzmed.ac.ir 3200319475328460029373 3200319475328460029373 Yes Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Pouran Karimi pouran.karimi@yahoo.com 3200319475328460029374 3200319475328460029374 No Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Javan 3200319475328460029375 3200319475328460029375 No Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Soheila Bani 3200319475328460029376 3200319475328460029376 No Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Saba Hoseini 3200319475328460029377 3200319475328460029377 No Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran Hamid Soltani-Zangbar 3200319475328460029378 3200319475328460029378 No Neuroscience Research Center, Tabriz University of Medical Sciences, Faculty of Advanced Medical Sciences, Tabriz, Iran Behnaz Sadeghzadeh-Oskouei 3200319475328460029379 3200319475328460029379 No Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran