en Others Others Review Review <div style="text-align: justify;"><strong>Introduction:</strong> The use of animals in experiments and their role in the development of medical sciences are undeniable. Humane endpoints terminate pain and distress in laboratory animals, which are experimented in painful procedures and an involuntary manner. This study was going to review studies published in this area to assist researchers in developing their approach. <strong>Methods:</strong> Articles used in this review study were obtained from relevant databases including Pubmed, Scopus, Science Direct, OVID, SID, Magiran and Google scholar. <strong>Results:</strong> &ldquo;Humane endpoints&rdquo; or killing the animal humanely means the point at which an experimental animal&rsquo;s pain and/or distress is terminated. This pain and distress are not necessarily accompanied by clinical symptoms and it can also be recognized by biochemical, physiological and molecular biomarkers testing. <strong>Conclusion:</strong> Regarding the extensive use of laboratory animals, the aim is not only to take care of animals but also to develop knowledge and prevent unintentional animal suffering and death. Increasing awareness of ethical issues regarding research animal use needs scientific information and designing experiments, which are terminated immediately after achieving main goals. Otherwise, it threatens the life of animal and leads to the animal suffering.</div> Laboratory animals, Humane endpoints, Animal welfare, Biomedical research. 1 6 http://ppj.phypha.ir/browse.php?a_code=A-11-963-8&slc_lang=en&sid=1 Houshang Najafi هوشنگ نجفی hnajafi@kums.ac.ir 3200319475328460029145 3200319475328460029145 No Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Reza Zarei رضا زارعی dr.ashtiyani@arakmu.ac.ir 3200319475328460029146 3200319475328460029146 No Student Research Committee, Arak University of Medical Sciences, Arak, Iran Abbas Alimoradian dr.alimoradian@arakmu.ac.ir 3200319475328460029147 3200319475328460029147 No Department of Pharmacology, School of Medicine, Arak University of Medical Sciences, Arak, Iran Mohaddeseh Asafari m.asafari@arakmu.ac.ir 3200319475328460029148 3200319475328460029148 No Deputy of Food and Drug, Arak University of Medical Sciences, Arak, Iran Mahsa Mohammadi dr.ashtiyani@arakmu.ac.ir 3200319475328460029149 3200319475328460029149 No Student Research Committee, Arak University of Medical Sciences, Arak, Iran Fatemeh Samadi dr.ashtiyani@arakmu.ac.ir 3200319475328460029150 3200319475328460029150 No Student Research Committee, Arak University of Medical Sciences, Arak, Iran Majid Ramezani m_ramezani@sina.tums.ac.ir 3200319475328460029151 3200319475328460029151 No Department of internal Medicine, School of Medicine, Baghitallah University of Medical Sciences, Teharn, Iran Saeed Changizi Ashtiyani dr.ashtiyani@arakmu.ac.ir 3200319475328460029152 3200319475328460029152 Yes Department of Physiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran