Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1400
6
1
gregorian
2021
9
1
25
3
online
1
fulltext
en
Protective effects of date palm pollen extract on gentamicin-induced hepatotoxicity
Gastrointestinal Physiology/Pharmacology
Gastrointestinal Physiology/Pharmacology
Experimental research article
Experimental research article
<div style="text-align: justify;"><strong>Introduction:</strong> Gentamicin, as an aminoglycoside antibiotic, is used to treat gram-negative bacterial infections. But despite its beneficial effects, gentamicin has side effects such as hepatotoxicity. Therefore, the aim of the present study was to investigate the protective effect of date palm pollen (DPP) hydroalcoholic extract against gentamicin-induced hepatotoxicity in rats. <strong>Methods:</strong> In present study the animals were divided into 5 groups, including control, sham, gentamicin and the two groups of gentamicin plus DPP extract at 200mg/kg and 400mg/kg. The plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzymes were measured to assess hepatic functional damages. Also, antioxidant enzymes activity including catalase (CAT) and superoxide dismutase (SOD) as well as total antioxidant capacity (FRAP) of the liver tissue sample were measured. Further, a tissue sample was fixed in 10% formaldehyde for hematoxylin and eosin staining and histopathological study. In the end of experiment, the animals were euthanized by deep anesthesia. <strong>Results:</strong> Gentamicin significantly increased the levels of plasma AST and ALT enzymes, caused histopathological damages, decreased CAT and SOD enzymes as well as FRAP in the liver tissue in comparison to the sham group. The concomitant administration of DPP hydroalcoholic extract and gentamicin with both examined doses could relatively improve these parameters, so that some parameters have not significant difference with the sham group. <strong>Conclusion: </strong>It can be concluded that the hydroalcoholic extract of DPP reduces histopathological damages, oxidative stress as well as hepatic enzymes following their increase by gentamicin.</div>
Gentamicin, Date palm pollen, Hepatotoxicity, Oxidative stress, Histopathological damages.
251
260
http://ppj.phypha.ir/browse.php?a_code=A-10-1418-2&slc_lang=en&sid=1
Zeynab
Mohamadi Yarijani
3200319475328460029296
3200319475328460029296
No
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Seyed Hamid
Madani
3200319475328460029297
3200319475328460029297
No
Molecular Pathology Research Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
Saeed
Changizi-Ashtiyani
3200319475328460029298
3200319475328460029298
No
Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Houshang
Najafi
hnajafi@kums.ac.ir
3200319475328460029299
3200319475328460029299
Yes
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran