en Renal Physiology/Pharmacology Renal Physiology/Pharmacology Experimental research article Experimental research article <div style="text-align: justify;"><span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">Phenylhydrazine (PHZ)-induced anaemia is associated with oxidative damage to erythrocytes and impaired renal function. Fresh palm oil (FPO) is a rich source of antioxidants and preserves renal function. This study investigated the possible reno-protective effects of FPO in PHZ-induced anaemic Wistar rats. </span><span class="fontstyle0"><strong>Methods:</strong> </span><span class="fontstyle2">Eighteen male Wistar rats were randomly assigned into normal control (NC), anaemic control (AC; 60mg/kg of PHZ) and anaemic treated (A+FPO; 60mg/kg of PHZ + 15% FPO diet) groups. PHZ was administered twice consecutively at 48h interval while FPO diet was given throughout the study period (14 days). </span><span class="fontstyle0"><strong>Results:</strong> </span><span class="fontstyle2">Erythrocyte count, haemoglobin concentration and haematocrit were increased in A+FPO compared with AC. FPO improved imbalances in the levels of glucose and electrolytes (Na</span><span class="fontstyle2" style="font-size:6pt;">+</span><span class="fontstyle2">, Cl</span><span class="fontstyle2" style="font-size:6pt;">-</span><span class="fontstyle2">, K</span><span class="fontstyle2" style="font-size:6pt;">+</span><span class="fontstyle2">, HCO</span><span class="fontstyle2" style="font-size:6pt;">3-</span><span class="fontstyle2">) in serum and urine of the anaemic rats. Urea and creatinine concentrations were decreased in serum and increased in urine in the A+FPO relative to AC. Proteinuria was decreased in A+FPO compared with AC. The levels of malondialdehyde, proinflammatory cytokines and caspase-3 were down-regulated in A+FPO compared with AC while interleukin-10 concentration, enzymatic antioxidants activities and total antioxidant capacity were up-regulated in A+FPO compared with AC. </span><span class="fontstyle0"><strong>Conclusion:</strong> </span><span class="fontstyle2">FPO demonstrated anti-anaemic effect and improved the impaired renal function associated with PHZ-induced anaemia.</span></div> Anaemia, Fresh palm oil, Haemolysis, Phenylhydrazine, Renal function 299 312 http://ppj.phypha.ir/browse.php?a_code=A-10-1447-1&slc_lang=en&sid=1 Ernest Atelhe Amama 3200319475328460031306 3200319475328460031306 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Augustine Lishilinimye Udefa augustineudefa@unical.edu.ng 3200319475328460031307 3200319475328460031307 Yes Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Favour Nyoh Beshel 3200319475328460031308 3200319475328460031308 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Efiok Aniekan Archibong 3200319475328460031309 3200319475328460031309 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Sunday Okpa 3200319475328460031310 3200319475328460031310 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Justina Nwandimma Nwangwa 3200319475328460031311 3200319475328460031311 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Ndianabasi Isidore Felix 3200319475328460031312 3200319475328460031312 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria Godwin Akronyi 3200319475328460031313 3200319475328460031313 No Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria