Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1400
9
1
gregorian
2021
12
1
25
4
online
1
fulltext
en
The cardiovascular responses after lipopolysaccharide microinjection into the dorsomedial periaqueductal gray in rats
Nervous system (others)
Others
Experimental research article
Experimental research article
<div style="text-align: justify;"><strong>Introduction:</strong> The dorsomedial periaqueductal gray (dmPAG) is located around the cerebral aqueduct with various functions such as cardiovascular regulation and is affected by inflammation. Lipopolysaccharide (LPS) is a complex molecule with an inflammatory effect that is known to affect blood pressure. In the present study, the cardiovascular effect of LPS microinjection into the dmPAG was investigated. <strong>Methods:</strong> Rats were divided into three groups consisting of 1: control; 2: 50 ng LPS and 3:100 ng LPS. Rats were mounted on a stereotaxic device after anesthesia and a continuous recording of cardiovascular parameters was done by a PowerLab device, connected to a cannulated femoral artery and drugs microinjected into dmPAG. The changes (Δ) in systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MA) and heart rate (HR) were calculated at different times and compared to the control group. <strong>Results:</strong> Both doses of LPS when microinjected into the dmPAG brought on a significant hypotensive response in the pressure parameters (MAP, SBP, and DBP) when compared to control. A non-significant increase in HR was also documented in both groups. <strong>Conclusion: </strong>The results of this experiment indicated that LPS, when microinjected into the dmPAG, induced a hypotensive response in anesthetized rats in both doses in comparison to control.</div>
Dorsomedial periaqueductal gray, Lipopolysaccharide, Inflammation, Blood Pressure
334
340
http://ppj.phypha.ir/browse.php?a_code=A-10-1463-1&slc_lang=en&sid=1
Reza
Mohebbati
3200319475328460029335
3200319475328460029335
No
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Reza
Nejad Shahrokh Abadi
3200319475328460029336
3200319475328460029336
No
Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Vida
Alikhani
3200319475328460029337
3200319475328460029337
No
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Naser
Shafei
shafeimn@mums.ac.ir
3200319475328460029338
3200319475328460029338
Yes
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran