en Renal Physiology/Pharmacology Renal Physiology/Pharmacology Experimental research article Experimental research article <div style="text-align: justify;"><span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">Renal ischemia/reperfusion injury (IRI) may influence distance organ such as lung. The severity of IRI induced- lung insult can be affected by gender and reperfusion time alteration. The aim of this study was to determine gender differences in renal IR inducedlung injury in different reperfusion time. </span><span class="fontstyle0"><strong>Methods:</strong> </span><span class="fontstyle2">Eighty male and female rats were assigned into 8 groups, 4 groups in each gender including: sham, renal ischemia for 45min by clamping renal vessels followed by 3, 24 or 48h reperfusion. Blood samples were obtained for measuring the serum level of blood urea nitrogen (BUN), creatinine (Cr), nitrite and malondialdehyde (MDA). The kidneys and lung tissues were removed and used for MDA and nitrite measurements and the histological changes evaluation. The lung water content was calculated. </span><span class="fontstyle0"><strong>Results:</strong> </span><span class="fontstyle2">In both genders, the rise in Cr and BUN reached the peak at 24h reperfusion. A significant increment was seen in female serum level of nitrite compared with males after 3h reperfusion. The renal MDA level of male increased during 3h reperfusion but not seen in females. The enhanced lung and renal tissues damages were depended to reperfusion time in both genders. The water content of lung was reduced in 3h of reperfusion groups. </span><span class="fontstyle0"><strong>Conclusion:</strong> </span><span class="fontstyle2">Sex effects and the time of reperfusion may be the important factors to consider clinical therapeutic of renal IRI as well as its impact on remote organs.</span></div> Renal ischemia-reperfusion, Lung, Gender, Distant organ 168 177 http://ppj.phypha.ir/browse.php?a_code=A-10-1485-1&slc_lang=en&sid=1 Fariba Azarkish 3200319475328460030928 3200319475328460030928 No Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Ali Atash Ab Parvar 3200319475328460030929 3200319475328460030929 No Department of Pathology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Mehdi Nematbakhsh 3200319475328460030930 3200319475328460030930 No Water and Electrolytes Research Center/Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran Aghdas Dehghani Aghdas.dehghani@hums.ac.ir 3200319475328460030931 3200319475328460030931 Yes Endocrinology and Metabolism Research Center/Department of Physiology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran