Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1401
3
1
gregorian
2022
6
1
26
2
online
1
fulltext
en
The effects of sildenafil on the hippocampal longterm potentiation in male rats
Blood and Immune System
Blood and Immune System
Experimental research article
Experimental research article
<div style="text-align: justify;"><span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">The hippocampal nitric oxide/cGMP signaling pathway plays a crucial role in memory processing. Phosphodiesterase interacts with this signaling pathway. There are controversial reports regarding the effect of sildenafil, a phosphodiesterase inhibitor, on learning and memory. Therefore, the effects of acute administration (intrahippocampal/ intra-dentate gyrus injection) of sildenafil on long-term potentiation (LTP) of rats were investigated. </span><span class="fontstyle0"><strong>Methods:</strong> </span><span class="fontstyle2">The rats were anesthetized with urethane and placed in a stereotaxic device for field potential recording. After ensuring a steady-state baseline response, a single intraperitoneal injection of saline or sildenafil (2 and10 µg/kg) was done. The population spike amplitude, the excitatory postsynaptic potentials (EPSPs) slope and paired-pulse stimuli (as an inhibitory interneuron) were compared between groups. </span><span class="fontstyle0"><strong>Results:</strong> </span><span class="fontstyle2">The results showed that population spike amplitude and EPSP slope significantly increased after sildenafil administration (10µg/kg) following titanic stimulation compared with the saline group. However, the sildenafil (2µg/kg) and control groups showed no difference regarding population spike amplitude and EPSP slope. Sildenafil had no significant effects on recurrent inhibition. </span><span class="fontstyle0"><strong>Conclusion:</strong> </span><span class="fontstyle2">The obtained results indicated that acute administration of sildenafil improved LTP via direct effects on the hippocampus of intact rats. Thus, sildenafil may enhance learning and memory processing by modulating the hippocampal synapse.</span></div>
Sildenafil, Phosphodiesterase inhibitor, Long term potentiation, Hippocampus
138
144
http://ppj.phypha.ir/browse.php?a_code=A-10-1336-3&slc_lang=en&sid=1
Siamak
Shahidi
3200319475328460030910
3200319475328460030910
No
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Mina
Vishteh
3200319475328460030911
3200319475328460030911
No
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Alireza
komaki
3200319475328460030912
3200319475328460030912
No
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Reihaneh
Sadeghian
sadeghian.r@skums.ac.ir
3200319475328460030913
3200319475328460030913
Yes
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran