en Blood and Immune System Blood and Immune System Experimental research article Experimental research article <div style="text-align: justify;"><span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">Methamphetamine (METH) addiction is an epidemic-growing problem globally. Studies confirmed a solid linkage between the prefrontal cortex (PFC) and drug seeking. The present study aimed to investigate PFC neural activity changes after injection of METH following METH-induced conditioned place preference (CPP) in the rats. </span><br /> <strong><span class="fontstyle0">Methods: </span></strong><span class="fontstyle2">After the development of CPP (0.5mg/kg METH for three days, SC), </span><span class="fontstyle3">in vivo </span><span class="fontstyle2">single-unit recordings were carried out the day after the post-test (post-conditioning day). On recording day, after stabilization and baseline recording (a 10-min period), the injection of METH (0.5mg/kg, SC) was performed and then, PFC neural activity was recorded for a 30-min period. </span><br /> <strong><span class="fontstyle0">Results: </span></strong><span class="fontstyle2">The results revealed that the injection of METH on the post-conditioning test significantly increases PFC neurons&rsquo; firing rate in animals that received METH during the CPP paradigm. </span><br /> <strong><span class="fontstyle0">Conclusion: </span></strong><span class="fontstyle2">It seems that maybe, PFC neurons appear to be implicated in the associated METH reward pathway and repeated exposure to METH affected the sensitivity of neurons in this area.</span></div> Reward system, Prefrontal cortex, Methamphetamine, Neuronal activity, Conditioned place preference 288 298 http://ppj.phypha.ir/browse.php?a_code=A-10-1513-2&slc_lang=en&sid=1 Zahra Taslimi 3200319475328460032679 3200319475328460032679 No Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Abdolrahman Sarihi 3200319475328460032680 3200319475328460032680 No Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran Sara Karimi 3200319475328460032681 3200319475328460032681 No Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Abbass Haghparast Haghparast@yahoo.com 3200319475328460032682 3200319475328460032682 Yes Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran