en Toxicology Toxicology Experimental research article Experimental research article <p><strong>Introduction: </strong>We investigated the subchronic toxicity concerns of a polyherbal formulation (PHF; Dr Iguedo Goko Cleanser&reg;) on haematological indices, brain and spleen histomorphology in exposed Wistar rats of both sexes.<br /> <strong>Methods:</strong> Thirty Wistar rats randomly allotted to six groups (5/group) were experimentally exposed to PHF via the oral route for 60 days as follows: control groups (1 and 4; given 5ml/kg distilled water); groups 2, 5 (476.24mg/kg) and 3, 6 (158.75mg/kg) body weight PHF, respectively. On 62nd day, animals were euthanized using carbon dioxide and sacrificed; spleen and brain tissues were evis-cerated, weighed and fixed in 10% buffered-formalin for histopathological assessment.<br /> <strong>Results:</strong> Our results showed significant increase in platelet in all experimental rats relative to control. Low dosed (158.75mg/kg) male rats recorded a significant increase in WBC relative to control. Also increased were MCV and MCH in male rats. High dosed female rats had increased RBC and MCV. Neutrophils and lymphocyte differentials were respectively decreased and increased in experimental groups relative to control. Histopathology of the spleen and brain tissues revealed degrees of pathologies like abnormal cytostructure of lymphoid follicle, degenerated T-lymphocytes, numerous organic deposits, degenerating neural cells, proliferating astrocytes, widely scattered blood vessel amongst others.<br /> <strong>Conclusion:</strong> Our findings revealed exposure-associated toxic effects of the quasi-drug formulation on blood parameters and histostructure of the spleen and brain. Findings suggest utmost caution on long-term use of the polyherbal formulation and avoidance whenever possible.</p> Drug compounding, Herbal medicine, Toxicology, Gliosis, Haematology 261 270 http://ppj.phypha.ir/browse.php?a_code=A-10-1700-1&slc_lang=en&sid=1 Godswill J. Udom godswill.udom@fuoye.edu.ng 3200319475328460033184 0000000337245944 Yes Department of Pharmacology and Toxicology, Faculty of Pharmacy, Federal University Oye-Ekiti, PMB 373, Oye-Ekiti, Nigeria Uduak P. Ise 3200319475328460033185 3200319475328460033185 No Department of Pharmacology and Toxicology, Faculty of Pharmacy, Bingham University, Karu, Nigeria Ayodeji Aturamu 3200319475328460033186 3200319475328460033186 No Department of Human Physiology, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria Moses A. Omoirri 3200319475328460033187 3200319475328460033187 No Department of Pharmacology and Toxicology, Faculty of Pharmacy, Federal University Oye-Ekiti, PMB 373, Oye-Ekiti, Nigeria Mba Ogbonnaya 3200319475328460033188 3200319475328460033188 No Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Akwa, Nigeria Israel K. Umana 3200319475328460033189 3200319475328460033189 No Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria Jude E. Okokon 3200319475328460033190 3200319475328460033190 No Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria