Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1402
6
1
gregorian
2023
9
1
27
3
online
1
fulltext
en
Toxicity assessment of a polyherbal drug on haematological parameters, brain and spleen histoarchitecture in exposed Wistar rats
Toxicology
Toxicology
Experimental research article
Experimental research article
<p><strong>Introduction: </strong>We investigated the subchronic toxicity concerns of a polyherbal formulation (PHF; Dr Iguedo Goko Cleanser®) on haematological indices, brain and spleen histomorphology in exposed Wistar rats of both sexes.<br />
<strong>Methods:</strong> Thirty Wistar rats randomly allotted to six groups (5/group) were experimentally exposed to PHF via the oral route for 60 days as follows: control groups (1 and 4; given 5ml/kg distilled water); groups 2, 5 (476.24mg/kg) and 3, 6 (158.75mg/kg) body weight PHF, respectively. On 62nd day, animals were euthanized using carbon dioxide and sacrificed; spleen and brain tissues were evis-cerated, weighed and fixed in 10% buffered-formalin for histopathological assessment.<br />
<strong>Results:</strong> Our results showed significant increase in platelet in all experimental rats relative to control. Low dosed (158.75mg/kg) male rats recorded a significant increase in WBC relative to control. Also increased were MCV and MCH in male rats. High dosed female rats had increased RBC and MCV. Neutrophils and lymphocyte differentials were respectively decreased and increased in experimental groups relative to control. Histopathology of the spleen and brain tissues revealed degrees of pathologies like abnormal cytostructure of lymphoid follicle, degenerated T-lymphocytes, numerous organic deposits, degenerating neural cells, proliferating astrocytes, widely scattered blood vessel amongst others.<br />
<strong>Conclusion:</strong> Our findings revealed exposure-associated toxic effects of the quasi-drug formulation on blood parameters and histostructure of the spleen and brain. Findings suggest utmost caution on long-term use of the polyherbal formulation and avoidance whenever possible.</p>
Drug compounding, Herbal medicine, Toxicology, Gliosis, Haematology
261
270
http://ppj.phypha.ir/browse.php?a_code=A-10-1700-1&slc_lang=en&sid=1
Godswill J.
Udom
godswill.udom@fuoye.edu.ng
3200319475328460033184
0000000337245944
Yes
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Federal University Oye-Ekiti, PMB 373, Oye-Ekiti, Nigeria
Uduak P.
Ise
3200319475328460033185
3200319475328460033185
No
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Bingham University, Karu, Nigeria
Ayodeji
Aturamu
3200319475328460033186
3200319475328460033186
No
Department of Human Physiology, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria
Moses A.
Omoirri
3200319475328460033187
3200319475328460033187
No
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Federal University Oye-Ekiti, PMB 373, Oye-Ekiti, Nigeria
Mba
Ogbonnaya
3200319475328460033188
3200319475328460033188
No
Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Akwa, Nigeria
Israel K.
Umana
3200319475328460033189
3200319475328460033189
No
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria
Jude E.
Okokon
3200319475328460033190
3200319475328460033190
No
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria