en Endocrine Physiology/Pharmacology Endocrine Physiology/Pharmacology Experimental research article Experimental research article <p><span style="line-height:1.6;"><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction: </strong>The consumption of fructose sweeteners as an alternative to high-calorie diets has been linked to various metabolic diseases due to increased oxidative stress. This study investigated changes in testicular and ovarian functions following Omega-3 polyunsaturated fatty acid supplementation (&omega;-3 PUFAs) in rats fed a High Fructose Diet (HFD).<br /> <strong>Methods: </strong>Forty Wistar rats were equally and randomly distributed into four groups. Each group was further subdivided by gender, with an equal number of animals in each sub-group. Group A assigned as control, while Groups B and C were administered HFD containing 10% and 20% v/v fructose solution, respectively. Group D rats were provided 20% v/v HFD and &omega;-3 PUFAs.&nbsp;<br /> <strong>Results: </strong>After six weeks of consumption, HFD resulted in reduced semen quality and gonadal reproductive hormone levels, while increasing testicular and ovarian oxidative stress. &omega;-3 PUFAs improved semen quality, reproductive hormone concentrations, and antioxidant defense system.<br /> <strong>Conclusion:</strong> HFD impairs gonadal function, however, dietary supplementation with &omega;-3 PUFAs improves gonadal functions by enhancing the antioxidant defense mechanism.</span></span></span></p> High-fructose diet,Infertility,Omega-3,Ovary,Oxidative stress 282 294 http://ppj.phypha.ir/browse.php?a_code=A-10-1724-1&slc_lang=en&sid=1 Ehitare Ekhoye ekhoye.ehitare@edouniversity.edu.ng 3200319475328460036218 0000000300329343 Yes Department of Physiology, College of Medical Sciences, Edo State University Uzairue, Nigeria Ejime Agbonifo-Chijiokwu eagbonifo-chijiokwu@delsu.edu.ng 3200319475328460036219 0000000301545515 No Department of Physiology, College of Health Sciences, Delta State University, Abraka, Nigeria Eze Nwangwa eknwangwa@delsu.edu.ng 3200319475328460036220 0000000301154119 No Department of Physiology, College of Health Sciences, Delta State University, Abraka, Nigeria