en Neurophysiology/Pharmacology Neurophysiology/Pharmacology Experimental research article Experimental research article <p style="text-align: justify;"><strong>Introduction:</strong> Acorus calamus Linn. from the Acoraceae family exhibits several benefits in neurological disorders but has not been studied for chronic constriction injury (CCI) of median nerve induced neuropathic pain. Damage to median nerve leads to work-related musculoskeletal disorders (WMSDs). this study aimed to assess the effects of the ethanolic root extract of Acorus calamus (EAC) on CCI-induced neuropathic pain and WMSDs in rats.<br /> <strong>Methods:</strong> Animals were randomly divided into 7 groups of 8 animals each. Group 1. Normal control, 2. Sham control, 3. CCI, 4. CCI+ vehicle (CMC), 5. CCI+gabapentin (50 mg/kg), 6. CCI+EAC (20 mg/kg), 7. CCI+EAC (40 mg/kg). On day 0, rats were subjected to the surgical procedure of exposure and ligation of the median nerve-produced CCI at the forearm level. Pain-sensitive tests (i.e., hot plate test, Randall Selitto test), and functional analysis (i.e., walking track) were performed. Total protein, lipid peroxidation, and histopathological changes were also estimated.<br /> <strong>Results:</strong> CCI significantly increased thermal and mechanical hyperalgesia, raised median functional index (walking track analysis), and induced biochemical and histological disruptions. Oral administration of EAC (40 mg/kg) and gabapentin (50 mg/kg) notably lowered CCI-induced nociceptive pain threshold, improved median nerve functional index, and mitigated tissue histological alterations.<br /> <strong>Conclusion: </strong>EAC has been found to decrease CCI-induced neuropathic pain of the median nerve. Its mechanisms likely involve neuroprotective, antioxidant, and anti-inflammatory properties.</p> Acorus calamus, Median nerve injury, Nerve functional index, Neuroprotection, Neuropathic pain, Walking track analysis 392 402 http://ppj.phypha.ir/browse.php?a_code=A-10-1747-1&slc_lang=en&sid=1 Shubhechha Bansod bansod.s1998@gmail.com 3200319475328460034200 0000000319377521 No Bharati Vidyapeeth (Deemed to be University) Poona College of Pharmacy, Pune Likhit Akotkar likhitakotkar254@gmail.com 3200319475328460034201 0000000342777806 No Bharati Vidyapeeth (Deemed to be University) Poona College of Pharmacy, Pune, India Subhash Bodhankar sbodh@yahoo.com 3200319475328460034202 0000000151682134 No Bharati Vidyapeeth (Deemed to be University) Poona College of Pharmacy, Pune, India Urmila Aswar aswarurmila@gmail.com 3200319475328460034203 0000000245667453 Yes Associate Professor, Bharati Vidyapeeth (Deemed to be University) Poona College of Pharmacy, Pune, India