en Toxicology Toxicology Short communication Short communication <strong>Introduction:</strong> Cyclophosphamide (CP) is the most common chemotherapy drug that has toxic effects on the male reproductive system. This experimental study aimed to assess the protective impact of saponin (SP) on the testicular damage induced via CP.<br /> <strong>Methods:</strong> Twenty-four male mice were separated randomly into 4 groups (N=6): 1. Control group, 2. CP, 3. SP and 4. CP + SP. CP (15mg/kg/weekly) and SP (2.5 mg/kg/daily) were intraperitoneally injected for 35 consecutive days. At the end of the study, the testicles were removed for the histological and biochemical (MDA, SOD, and GPX) evaluation.<br /> <strong>Results: </strong>CP induced degenerative changes in testicular tissues and SP treatment reduced these alterations. The results indicated that CP increased MDA levels and reduced SOD, GPX, and testosterone levels (P<0.05) in contrast to the control group. In the CP+SP group, a significant reduction (P<0.01) in MDA levels and the elevation of SOD, GPX, and testosterone levels were observed.&nbsp;<br /> <strong>Conclusion:</strong> It seems saponin may reduce CP-induced reproductive toxicity in male mice.&nbsp; Cyclophosphamide,Oxidative stress,Saponin,Testis 3 9 http://ppj.phypha.ir/browse.php?a_code=A-15-1874-3&slc_lang=en&sid=1 Mojgan Timar mojitimar.st@gmail.com 3200319475328460034146 0000000310549235 No Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Ramin Salimnejad r.salimnegad67@gmail.com 3200319475328460034147 000000027437502X No Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Mohammad Ghasem Golmohammadi golmohammadi50@gmail.com 3200319475328460034148 0000000284546558 No Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Shokofeh Banaei s.banaei75@gmail.com 3200319475328460034149 0000000287423895 Yes Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Zahra Mehraban zahraob81@yahoo.com 3200319475328460034150 0000000255324219 No Department of Midwifery, Ardabil Branch, Islamic Azad University, Ardabil, IR, Iran