Physiology and Pharmacology

en Corrigendum to “The evaluation of synergistic effects of combination therapy with sulfasalazine and angiotensin-converting enzyme inhibitor in the treatment of experimental colitis in mice” [Physiol Pharmacol 28 (2024) 169-179] Gastrointestinal Physiology/Pharmacology Gastrointestinal Physiology/Pharmacology Experimental research article Experimental research article Aims: Intestinal colitis or ulcerative colitis is an inflammatory bowel disease that causes long-term inflammation and ulcers in the gastrointestinal tract. It has been suggested that mucosal expression of angiotensin II (AT-II) is increased in colitis. Here, we examined the potential therapeutic effects of combination therapy regarding Enalapril, as an Angiotensin-converting enzyme inhibitor, with sulfasalazine (SSZ) in a murine colitis model. Methods: Male C57BL/6 mice divided to five groups: control group (distilled water), dextran sulphate sodium (DSS) (colitis group) (1 % DSS), SSZ (positive control group) with 100 mg/ kg/day, Enalapril alone group with 4 mg/kg/day, Enalapril (4 mg/kg/day) + SSZ (100 mg/kg/day). Results: There was a significant reduction in disease activity index among the group of mice receiving the combination of Enalapril and SSZ compared to the colitis group. Enalapril and SSZ treatment was associated with a lower reduction in colon length, decreased colon weight, spleen weight and spleen-to-body weight in mice with colitis. Following DSS administration, Enalapril and SSZ also significantly decreased MDA levels as an oxidant marker, and increased total thiol, SOD, and CAT levels, as anti-oxidants. In addition, mucosal damage, crypt loss, pathological changes, and inflammation score decreased after treatment with Enalapril and SSZ in comparison with colitis group. The combination of Enalapril and SSZ reduced colon collagen content and caused fibrosis to decrease in comparison with colitis group.  Conclusion: The results of this study indicated that Enalapril alone and in combination with SSZ decreased inflammation and clinical symptoms of colitis induced by DSS.   Colitis, Angiotensin-converting enzyme inhibitor, Enalapril, inflammation 494 495 http://ppj.phypha.ir/browse.php?a_code=A-10-1946-2&slc_lang=en&sid=1 Asma Mostafapour MostafapourA1@mums.ac.ir 3200319475328460038144 3200319475328460038144 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Fereshteh Asgharzadeh Asgharzadehyf@mums.ac.ir 3200319475328460038145 3200319475328460038145 No Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Seyedeh Elnaz Nazari nazarie4003@mums.ac.ir 3200319475328460038146 3200319475328460038146 No Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Moein Eskandari EskandariM982@mums.ac.ir 3200319475328460038147 3200319475328460038147 No Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Niloufar Naghibzadeh Naghibzadehn951@mums.ac.ir 3200319475328460038148 3200319475328460038148 No Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Javad baharara Bahararaj@gmail.com 3200319475328460038149 3200319475328460038149 No Department of Biology & Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Amir Avan avana@mums.ac.ir 3200319475328460038150 3200319475328460038150 No Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Seyed Mahdi Hassanian HASANIANMEHRM@MUMS.AC.IR 3200319475328460038151 3200319475328460038151 No Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Majid Khazaei khazaeimaj@gmail.com 3200319475328460038152 0000000279795699 Yes Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran