en Gastrointestinal Physiology/Pharmacology Gastrointestinal Physiology/Pharmacology Experimental research article Experimental research article <strong>Introduction: </strong>Eryngium billardieri has been demonstrated in previous studies to possess anti-inflammatory, antioxidant, and wound-healing properties. Colitis, an inflammatory bowel disease with unknown causes, often leads to numerous side effects associated with current medications. Therefore, this study aimed to investigate the anti-ulcerative potential of E. billardieri extracts in experimental colitis.<br /> <strong>Methods:</strong> The hydroalcoholic extract of E. billardieri and its aqueous and ethyl acetate partitions were prepared using the maceration method, and the polyphenol content was determined for each extract. Male Wistar rats with acetic acid-induced colitis were orally administered three different doses (50, 100, 200 mg/kg) of the extract and each partition for 5 consecutive days. On the sixth day, the rats&rsquo; colons were removed and analyzed for macroscopic parameters (ulcer index), microscopic parameters (total colitis index), as well as inflammatory and oxidative stress markers, including myeloperoxidase and malondialdehyde, respectively.<br /> <strong>Results: </strong>The total phenol content for the dry extract and aqueous and ethyl acetate partitions were 6.51, 4.15, and 8.59 mg gallic acid equivalent/g, respectively. The hydroalcoholic extract and ethyl acetate partition at all three examined doses were able to significantly alleviate most parameters related to colitis. However, the aqueous partition did not improve most of the colitis features except for the tissue level of malondialdehyde.<br /> <strong>Conclusion:</strong> The study concludes that the total extract of E. billardieri, as well as the ethyl acetate partition, exhibited anti-colitis properties in a dose-related manner. It is suggested that the effective substances responsible for these properties are non-polar compounds that are not extracted by aqueous partitioning. Further studies are needed to identify and characterize these effective compounds.<br /> <span style="font-size:10pt"><span style="line-height:200%"><span courier="" new="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:200%"><span courier="" new="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span> Anti-inflammatory, Ulcerative colitis, E. billardieri, Plant extract, Rats 56 65 http://ppj.phypha.ir/browse.php?a_code=A-10-855-1&slc_lang=en&sid=1 Zahra Heydari Zahraheydari9675@gmail.com 3200319475328460034174 0009000106469083 No Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Masoud Sadeghi-Dinani m_sadeghi@pharm.mui.ac.ir 3200319475328460034175 0000000155653879 No Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Ardeshir Talebi talebi@med.mui.ac.ir 3200319475328460034176 0000000173135086 No Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mohsen Minaiyan minaiyan@pharm.mui.ac.ir 3200319475328460034177 0000000221296299 Yes Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran