en بررسی اثر تخریب اختصاصی مسیر شکمی- جانبی PAG به NRM توسط نوروتوکسین Ibotenic Acid در آزمون فرمالین Nervous system (others) Others Experimental research article Experimental research article It has been reported that neurons in the VL PAG projecting to nucleus raphe magnus (NRM) utilize excitatory amino acids as neurotransmitter. This projection plays an important role in the descending pain modulatory system. In order to determine the role of this pathway in pain perception, male rats received unilateral injection of ibotenic acid stereotaxically. For lesioning VL PAG, unilateral injection of ibotenic acid (0.2 and 0.5 µl) (a specific neurotoxin for EAA-containing neurons) was made. After a week recovery period, the nociception was evaluated using formalin test for a period of 60 min. At the end of experiments, animals were perfused by formaldehyde 10%. Serial sections (80 µm) wee prepared using vibratome. The sections were then Nissl stained and examined to determine the lesion location. The results revealed a significant increase in the first phase of formalin test. However, in the second phase, only 0.5 µl of the neurotoxin caused a significant decrease in pain perception. It is concluded that NMDA receptors within the PAG are involved in the perception of pain as measured in the formalin test. Excitatory amino acids; Analgesia; NRM, VL PAG; Formalin test 213 218 http://ppj.phypha.ir/browse.php?a_code=A-10-70-2&slc_lang=en&sid=1 Farnaz Nikbakht 3200319475328460011655 3200319475328460011655 Yes Neuroscience Research Center, Shaheed Beheshti Univ. Med. Sci., Tehran, Iran Zhila Behzadi 3200319475328460011656 3200319475328460011656 No