en Nervous system (others) Others Experimental research article Experimental research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction:</strong> Spermatogenesis is a complex physiological process susceptible to various influencing factors, among which scrotal hyperthermia is noteworthy. This investigation assesses the impact of conditioned medium derived from Sertoli cells on spermatogenic activity within the testicular tissue of adult male mice after exposure to scrotal hyperthermia.<br /> <strong>Methods: </strong>A cohort of 40 adult male NMRI mice was employed in this research, and they were allocated ran-domly into four distinct groups as follows: (1) the control group comprising untreated animals, (2) the hyperthermia group subjected to scrotal hyperthermia through immersion in water at a tempera-ture of 43&deg;C for 20 minutes, administered five times every other day, (3) the DMEM group receiv-ing 10&mu;l of Dulbecco&rsquo;s Modified Eagle Medium (DMEM), and (4) the Sertoli cell-conditioned me-dium (SCCM) group, administered 10&mu;l of SCCM. After the induction of hyperthermia, intraperito-neal injections of SCCM were administered to the mice daily for 35 days. Samples of sperm were gathered from the epididymal tail, and the testis tissue was subsequently harvested for histological examination and molecular analysis.<br /> <strong>Results:</strong> Our findings revealed that the administration of SCCM yielded significant increases in the quanti-ties of sperm and germ cells compared to the other groups. Additionally, the relative gene expres-sion levels of receptor tyrosine kinase (C-kit), stimulated by retinoic acid 8 (Stra8), and proliferat-ing cell nuclear antigen (Pcna) exhibited a substantial elevation within the SCCM group compared to the other experimental groups.<br /> <strong>Conclusion: </strong>The utilization of SCCM holds promise as a therapeutic intervention for addressing infertility con-cerns, and it presents potential applications within the realms of the field of reproductive and re-generative medicine.</span></span></p> Sertoli cell-conditioned medium,Spermatogenesis,Scrotal hyperthermia 293 305 http://ppj.phypha.ir/browse.php?a_code=A-10-2262-1&slc_lang=en&sid=1 Reza Soltani rezasoltanikazemii@gmail.com 3200319475328460038042 3200319475328460038042 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hojjat-Allah Abbaszadeh hoomanabs@gmail.com 3200319475328460038043 3200319475328460038043 No Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohsen Norouzian norozian93@gmail.com 3200319475328460038044 3200319475328460038044 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Fakhroddin Aghajanpour fakhroddin72@gmail.com 3200319475328460038045 3200319475328460038045 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Azar Afshar azar.afshar8894@gmail.com 3200319475328460038046 3200319475328460038046 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Abbas Aliaghaei aghaei60@gmail.com 3200319475328460038047 3200319475328460038047 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Nafiseh Moeinian moeinian.ptu@gmail.com 3200319475328460038048 3200319475328460038048 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ali Dehghani nejad amirali3532@gmail.com 3200319475328460038049 3200319475328460038049 No Department of Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran Fatemeh Fadaei Fathabadi rezaneshat1995@gmail.com 3200319475328460038050 0009000541248306 Yes Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad-Amin Abdollahifar m_amin58@yahoo.com 3200319475328460038051 0000000169473285 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran