en Cardiovascular Physiology/Pharmacology Cardiovascular Physiology/Pharmacology Experimental research article Experimental research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction:</strong> Hypercholesterolemia is a condition in which the blood contains elevated levels of low-density lipoprotein (LDL) and non-high-density lipoprotein (HDL). There are varieties of different diets used by different laboratories as a recipe for induction with varying levels of hypercholesterolemia. This study aims to investigate the role of cholic acids in enhancing hypercholesterolemia parameters in Sprague Dawley rats.<br /> <strong>Methods: </strong>Nine Sprague Dawley rats (250 g &plusmn; 50 g BW) were used to investigate the most effective diet that is cost-effective for inducing hypercholesterolemia. The rats were randomly divided into 3 groups: normal diet (ND) (n=3), high cholesterol diet (HCD 1), a combination of 2% cholesterol and 0.5% cholic acid (n=3), and high HCD 2, a combination of 2% cholesterol and 30% ghee (n=3). After 4 weeks of feeding, blood samples were collected for lipid profiling, which included total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL). The liver, kidney, and brain were removed for histopathological examination using hematoxylin and eosin (H&E) staining.<br /> <strong>Results:</strong> The lipid profile measurements show significant differences between the HCD 1 group for total cholesterol, LDL cholesterol, non-HDL cholesterol, and total cholesterol/ HDL ratio compared to the normal group. HCD 2 shows no significant changes in lipid profiles compared to the normal group.<br /> <strong>Conclusion: </strong>Cholic acid helps in the absorption of cholesterol and enhances the hypercholesterol parameters in diet-induced SD rats based on lipid profile analysis and histology of the liver and kidney.</span></span></p> Dietetical recipe,Hypercholesterolemia,Low-density lipoprotein,SD rats 400 408 http://ppj.phypha.ir/browse.php?a_code=A-10-2279-1&slc_lang=en&sid=1 Azmina Hassan nurazminahassan@gmail.com 3200319475328460038124 3200319475328460038124 No School of Health Sciences, Health Campus Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia Zuraidah Abdullah zuraidahabdullah@usm.my 3200319475328460038125 3200319475328460038125 No School of Health Sciences, Health Campus Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia Niu Jin Tan niujinmy@gmail.com 3200319475328460038126 3200319475328460038126 No ZN Abqary Enterprise, 43-1 Jalan DU2/1, Taman Damai Utama, 47180 Puchong, Selangor, Malaysia Sabreena Safuan sabreena@usm.my 3200319475328460038127 0000000279248425 Yes School of Health Sciences, Health Campus Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia