en Endocrine Physiology/Pharmacology Endocrine Physiology/Pharmacology Experimental research article Experimental research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction:</strong> The objective of the study was to find out the influence of Thioflavin-T (ThT) on obesity and fatty liver by investigating the adipokine hormones, and insulin serum level of male NMRI mice which were exposed to a high-fat diet (HFD).<br /> <strong>Methods:</strong> 50 adult male NMRI mice were separated into five groups: n=10. The control group was given a standard diet at twelve-week intervals. The sham group was nourished with HFD that lasted for 8 weeks, afterwards, the group received a standard diet and solvent water (0.5ml) by gavage (4 weeks). The experimental groups 1-3 were nourished with HFD (4% cholesterol, 60% fat) eight-week period. Then, the treatment period started in experimental groups by receiving a normal diet in addition to ThT with three doses (5,10 and 15 mg/kg, 0.5ml), via gavage (4 weeks).&nbsp;<br /> <strong>Results: </strong>HFD contributed to a substantial reduction in serum adiponectin levels and increased leptin serum in the sham group opposite to the control group (P< 0.001). However, the concentration of both adipokine hormones was significantly modified under the treatment of ThT in a dose-dependent manner. Insulin serum increased in the sham group significantly (P< 0.001), meanwhile, a significant decrease was shown in experimental groups 2, and 3 than in the sham group (P<0.01). ThT also reduced HOMA-IR in experimental groups. The introduction of ThT in varying doses led to the induction of polymorphonuclear cells in the liver tissue.<br /> <strong>Conclusion: </strong>Our findings propose that ThT can affect liver function and body weight by modulating the serum levels of adipokine hormones besides decreasing the level of insulin and HOMA-IR in mice fed with HFD.</span></span></p> Adiponectin, Leptin, Fatty liver, Obesity, Thioflavin-T 409 418 http://ppj.phypha.ir/browse.php?a_code=A-10-2310-1&slc_lang=en&sid=1 Nafiseh Amani-Ekhtesar amaniekhtesar@yahoo.com 3200319475328460038128 3200319475328460038128 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran parichehreh yaghmaei yaghmaei_p@yahoo.com 3200319475328460038129 0000000198886935 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran azadeh ebrahim habibi azadehabibi@yahoo.fr 3200319475328460038130 3200319475328460038130 No Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran leyla karkhaneh l_karkhaneh@yahoo.com 3200319475328460038131 0000000247362215 Yes Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran