en Neurophysiology/Pharmacology Neurophysiology/Pharmacology Experimental research article Experimental research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction: </strong>Methamphetamine (MA) addiction is a major global public health concern, yet there is currently no approved medication that effectively treats this addiction. Kisspeptin is a neuropeptide that has a role in the reproductive system, metabolism and energy balance, and metastasis suppression in different types of cancers. The kisspeptin receptors, GPR54 are widely distributed in the brain&rsquo;s memory-related structures. Previous studies have revealed that the opioid system contributes to the addictive effects of MA. Additionally, preclinical studies have shown that a derivative of kisspeptin possesses anti-opioid properties. This study aimed to clarify the role of kisspeptin-13 (KP-13) on reward and reinstatement-related memory associated with MA in the conditioned place preference test.<br /> <strong>Methods:</strong> We evaluated pre-treatment with intracerebroventricular KP-13 for 3 consecutive days (2.5 &mu;l/nostril, once a day) in a conditioned place preference test induced by MA. MA was administered intraperitoneally at a dose ranging from 1 to 7 mg/kg, once daily, beginning at 1 mg/kg on day one and increasing by 1 mg/kg per day up to the day 7.<br /> <strong>Results: </strong>We found that KP-13 suppresses the reward behavior in MA-treated rats, while it has no significant effect on reinstatement behavior after one week of MA cessation, which could be attributed to the ineffective dose of KP-13.<br /> <strong>Conclusion: </strong>The findings indicate that KP-13 alters the rewarding and motivating effects of MA. Further research involving both multiple and single administrations of KP-13 before the reinstatement test is necessary to throw light on its impact on withdrawal-related reinstatement.</span></span></p> Kisspeptin-13, Methamphetamine, Reward, Reinstatement, Condition place preference 146 154 http://ppj.phypha.ir/browse.php?a_code=A-10-2237-2&slc_lang=en&sid=1 Somayeh Nazari snazari70@yahoo.com 3200319475328460037741 3200319475328460037741 No Student Research Committee, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran Mobina Gheibi mobinagheibi78@gmail.com 3200319475328460037742 3200319475328460037742 No Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran Hamed Ghazvini hghazvini1@gmail.com 3200319475328460037743 3200319475328460037743 No Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran Raheleh Rafaiee rachel.rafaie@yahoo.com 3200319475328460037744 3200319475328460037744 No Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran seyedeh masoumeh Seyedhosseini Tamijani m.seyedhoseini@mazums.ac.ir 3200319475328460037745 3200319475328460037745 Yes Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran