en Endocrine Physiology/Pharmacology Endocrine Physiology/Pharmacology Experimental research article Experimental research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction: </strong>Ovarian torsion is an emergency condition that occurs when the adnexa undergoes complete or partial rotation. Following ovarian torsion, damage caused by ischemia/reperfusion can impact fertility and sex hormone secretion. Our study aims to investigate the role of safranal in preventing ovarian ischemia-reperfusion injury in rats.<br /> <strong>Methods: </strong>Animal subgroups included: sham, ovarian torsion/detorsion (OT), ovarian torsion/detorsion with the treatment of safranal (OTS 0.1, 0.5), and safranal group (OS 0.1, 0.5). Ovarian torsion was induced in the left ovary of rats for 2 hours. Intraperitoneal treatment with safranal at 0.1 and 0.5 mg/kg doses was performed 30 minutes before the detorsion operation. 48 h after detorsion, ovarian tissue was collected to evaluate the histopathological scores and apoptosis gene expression of Bcl-2, caspase 3, and Bax. Blood samples were collected to measure plasma estradiol levels and oxidative stress parameters.<br /> <strong>Results: </strong>Analyses demonstrated that ovarian follicular damage was accompanied by increased serum malondialdehyde (MDA) levels and increased expression of Bcl2 and caspase-3 genes. Additionally, serum levels of glutathione peroxidase (GPX), estrogen, and superoxide dismutase (SOD) decreased in the OT group. However, treatment with safranal 0.5 mg/kg was accompanied by an improvement in the histopathological score of the ovarian tissue and a reduction in apoptosis and oxidative stress.<br /> <strong>Conclusion: </strong>Safranal exhibits antioxidant properties in a rat model of ovarian torsion and could be considered a cost-effective therapeutic option for ovarian detorsion in gynecological clinics.&nbsp;</span></span></p> Safranal,Oxidative Stress,Ischemia-reperfusion,Ovaries 184 193 http://ppj.phypha.ir/browse.php?a_code=A-10-2328-1&slc_lang=en&sid=1 Marzieh Ebrahimi Marzieh.ebr74@gmail.com 3200319475328460037761 3200319475328460037761 No Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran Masoumeh Fani m.fani2017@yahoo.com 3200319475328460037762 3200319475328460037762 No Department of Anatomical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Seyed-Hosein Abtahi-Evari Abtahi.h@gmu.ac.ir 3200319475328460037763 3200319475328460037763 No Department of Biochemistry, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Balal Brazvan bbarazvan@gmail.com 3200319475328460037764 3200319475328460037764 No Department of Anatomical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Sohrab Azin Sohrab.azin.75119@gmail.com 3200319475328460037765 3200319475328460037765 No Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran Majid Shokoohi a.shokoohy@yahoo.com 3200319475328460037766 3200319475328460037766 No Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Sajjad Abbasi Sajjadabbasi75@gmail.com 3200319475328460037767 3200319475328460037767 No Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran vida Alikhani vd.alikhani@gmail.com 3200319475328460037768 3200319475328460037768 No Department of Physiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Amir- hosein Ebadi ebadi.amirhossein@hotmail.com 3200319475328460037769 3200319475328460037769 No Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran Malihe soltani soltani.m@gmu.ac.ir 3200319475328460037770 3200319475328460037770 No Department of Anatomical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Maryam Moghimian moghimian.m@gmu.ac.ir 3200319475328460037771 3200319475328460037771 Yes Department of Physiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran