en Blood and Immune System Blood and Immune System Clinical research article Clinical research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction: </strong>Fatigue is a common issue among the elderly with chronic illnesses. This pilot study examined the effects of hemoglobin, age, Sirtuin1 (SIRT1), and rs7069102 on fatigue in older persons with chronic illness in Indonesia.<br /> <strong>Methods:</strong> A cross-sectional study of 129 elderly individuals with chronic illnesses was conducted using a moderated mediation analysis. A questionnaire was used to evaluate fatigue, SIRT1 levels were measured using ELISA, and the genotypes of rs7069102 were identified using PCR-CTPP. Hayes&rsquo; approach was used in both the mediation and moderated mediation studies.<br /> <strong>Results: </strong>Hemoglobin levels were negatively correlated with fatigue (r = -0.182, p = 0.038), but age was positively correlated with fatigue (r = 0.183, p = 0.039). The mediation analysis revealed that age had no significant effect on the relationship between hemoglobin level and fatigue. However, moderated mediation analysis indicated a significant indirect effect of the rs7069102 GG genotype on the relationship between hemoglobin, age, and fatigue at all SIRT1 levels (&beta;(mean-1SD) = -0.100, p = 0.010; &beta;(mean) = -0.112, p = 0.007; &beta;(mean+1SD) = -0.122, p=0.006).<br /> <strong>Conclusion: </strong>This study shows that the rs7069102 GG genotype at all SIRT1 plasma levels acts as a mediator, reducing the effect of age-related hemoglobin on fatigue. These findings indicate the intricate interaction between genetic factors, physiological parameters, and fatigue perception in these individuals, suggesting further investigation.</span></span></p> Fatigue, Hemoglobin, SIRT1, SNP rs7069102, Elderly 240 250 http://ppj.phypha.ir/browse.php?a_code=A-10-2381-1&slc_lang=en&sid=1 Dedi Ardinata dedi1@usu.ac.id 3200319475328460037964 0000000272144295 Yes Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatra, Indonesia Novita Sari Harahap novitahrp@unimed.ac.id 3200319475328460037965 3200319475328460037965 No Department of Sport Science, Faculty of Sport Science, Universitas Negeri Medan, Medan, North Sumatra, Indonesia Nenni Dwi Aprianti Lubis nenni@usu.ac.id 3200319475328460037966 3200319475328460037966 No Department of Nutrition, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatra, Indonesia Tetty Aman Nasution tetty@usu.ac.id 3200319475328460037967 3200319475328460037967 No Department of Microbiology, Universitas Sumatera Utara, Medan, North Sumatra, Indonesia