en Toxicology Toxicology Experimental research article Experimental research article <p><span style="color:#000000;"><span style="font-size:12px;"><span style="font-family:Arial;"><strong>Introduction: </strong>Di-2-ethylhexyl phthalate (DEHP), a chemical used in medical bags and tubing, can leach into the bloodstream and bone marrow, causing oxidative stress in bone marrow mesenchymal stem cells (BMSCs). This study investigates the potential of gallic acid (GA), a potent antioxidant, to counteract DEHP-induced oxidative effects in vitro.<br /> <strong>Methods: </strong>BMSCs were exposed to various GA concentrations (0.063, 0.125, 0.25, 1, and 10 &mu;M) for 4 days, identifying 0.25 &mu;M GA as the optimal concentration based on cell viability. Cells were then treated with 100 and 500 &mu;M DEHP, with or without 0.25 &mu;M GA, for 4 or 8 days to assess viability, population doubling number, total protein levels, malondialdehyde levels, total antioxidant capacity, catalase and superoxide dismutase activity, cellular morphology, and expression of Nrf2 and NF&kappa;B genes.<br /> <strong>Results:</strong> Treatment with DEHP led to a significant, dose- and time-dependent reduction in viability, proliferation rate, total protein levels, total antioxidant capacity, and the activity of antioxidant enzymes. In contrast, GA treatment significantly increased these parameters. Additionally, DEHP resulted in an increase in malondialdehyde levels and caused morphological changes as well as down-regulated NF&kappa;B and up-regulating Nrf2 expression. In the co-treatment group, GA counteracted the toxic effects of DEHP at 100 &mu;M compared with control, whereas it only partially mitigated the toxic effects at 500 &mu;M.<br /> <strong>Conclusion:</strong> Consuming low concentrations of GA over time may reduce DEHP-induced oxidative stress. Further animal studies with GA-rich foods are essential to confirm its preventive benefits in vivo.</span></span></span><span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><span style="font-size:14.0pt"><span style="line-height:150%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></p> Gallic acid,Di-2-ethylhexylphthalate,Bone marrow,mesenchymal stem cells,Oxidative stress. 110 124 http://ppj.phypha.ir/browse.php?a_code=A-10-432-5&slc_lang=en&sid=1 Mohammad Husein Abnosi m-abnosi@araku.ac.ir 3200319475328460039116 0000000214858847 Yes Arak University Mahrokh Parvaz mozhganb14194@yahoo.com 3200319475328460039117 3200319475328460039117 No Arak University