en Cell, Stem Cell and Cancer Cell, Stem Cell and Cancer Experimental research article Experimental research article <span style="font-size:12px;"><span style="font-family:Arial;"><span style="line-height:200%"><span new="" roman="" times=""><b><span style="line-height:200%">Background:</span></b><span style="line-height:200%"> Medicinal plants perform a prominent function in improving sustainable healthcare system and benefits because of enriching bioactive components in pharmacology industry. The aim for present research is to explore potential medicinal attributes of <i>Teucrium polium L.</i> essential oil (TEO), focusing on antioxidant, anticancer and antimicrobial attributes. </span></span></span><br /> <span style="line-height:200%"><span new="" roman="" times=""><b><span style="line-height:200%">Methods:</span></b><span style="line-height:200%"> Initially, TEO isolated by distillation method and existence substances such as spathulenol (12.98 %), beta-pinene (10.07 %) and germacrene B (9.21 %) identified using gas chromatography. Functional groups detected in TEO, which confirmed through Fourier transform infrared spectroscopy. </span></span></span><br /> <span style="line-height:200%"><span new="" roman="" times=""><b><span style="line-height:200%">Results:</span></b><span style="line-height:200%"> In addition, total phenol and flavonoids were reported 198.1 &plusmn; 4.19 mg gallic acid equivalent (GAE)/g dry weight (DW) and 56.23 &plusmn; 9 mg rutin (R)E/g DW, respectively. The promising antioxidant function of TEO was proved using achieved IC₅₀ from 2,2-diphenyl-1-picrylhydrazyl (94.35 &plusmn;3.2 &mu;g/mL), 2,2&#39;-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (83.3 &plusmn; 5.1 &mu;g/mL) and ferric ions (Fe³⁺) reducing antioxidant power (2.8 &plusmn; 0.02 &mu;g ascorbic acid equivalent (AAE)/g). Additionally, an acceptable cytotoxicity detected by TEO according to obtained results. The lowest (1.8 mm) and highest (13.8 mm) inhibition zones for TEO were observed against </span><i><span style="line-height:200%">Escherichia coli</span></i><span style="line-height:200%"> (<i>E. coli</i>) at a concentration of 20 mg/L and</span><i><span style="line-height:200%"> Staphylococcus aureus</span></i> <span style="line-height:200%">at 80 mg/L level in disk diffusion agar, respectively. The structure of <i>E. coli</i> depicted using a scanning electron microscopy evaluation after treating by TEO, which indicated several effects on membrane and denaturalization for bacterial cell. </span></span></span><br /> <span style="line-height:200%"><span new="" roman="" times=""><b><span style="line-height:200%">Conclusion:</span></b><span style="line-height:200%"> Overall, significant antimicrobial, antioxidant and anticancer functions of TEO suggested a candidate for further research in pharmacology industry and novel therapeutic agents.</span></span></span><br /> &nbsp;</span></span> ,Antimicrobial,Antioxidant,Anticancer,Essential oil,Teucrium polium L. 0 0 http://ppj.phypha.ir/browse.php?a_code=A-10-2567-1&slc_lang=en&sid=1 Samira Moradi Samira.moradi@ut.ac.ir 3200319475328460037216 3200319475328460037216 No Department of Food Science and Technology, Bioprocessing and Biodetection Laboratory, University of Tehran, Karaj, Iran Marjan Nouri marjan.nouri@ut.ac.ir 3200319475328460037217 3200319475328460037217 Yes Department of Food Science and Technology, Ro. C., Islamic Azad University, Roudehen, Iran