en Cell, Stem Cell and Cancer Cell, Stem Cell and Cancer Experimental research article Experimental research article <p><span style="font-size:12px;"><span style="font-family:Arial;"><span style="line-height:2;"><b>Background:</b> Peripheral nerves have limited self-restoration ability, but targeted nerve regeneration can be increased by growth factors and stem cells. This study investigated the effects of umbilical cord stroma stem cells grown on nano poly lactic-co-glycolic acid (PLGA) scaffolds of <i>Cichorium intybus</i> L. to repair severed sciatic nerve of male Wistar rats.<br /> <b>Materials methods: </b>Isolation of umbilical cord stroma stem cells, and primary culture was done. After extracting from <i>Cichorium intybus</i> L., a PLGA scaffold was fabricated. 45 male Wistar rats (230-260 g) were divided into 9 groups (<b>G1</b>= healthy control, <b>G2</b>=sciatic nerve injury(sni), <b>G3</b>=sni treated with umbilical cord cells(ucc), G4= snj treated with <i>Cichorium intybus</i> L(cil), <b>G5</b>= snj treated with cil.+ ucc, <b>G6</b> = snj treated with cil +PLGA scaffold,<b> G7=</b>snj with PLGA scaffold, <b>G8</b>= snj treated with PLGA scaffold+ucc,<b> G9=</b> snj treated with cil + PLGA scaffold+ ucc. After eight weeks of treatment, the evaluation of sciatic nerve damage repair included electrophysiological, sciatic functional index and Other indicators</span><span style="line-height:normal"><span style="line-height:2;">&nbsp;examination.<br /> <b>Results: </b>In the group treated with <i>cil.</i>+PLGA +ucc, sciatic nerve repair was observed with an increase in nerve conduction velocity (m/s), amplitude muscle action potential (mv), number of (vessels and fiber), most significant area of vessels, myeline, axon, and fiber (1000 micro m2) and a decrease in statistic functional index (0 to -50) (P<0.0001).<br /> <b>Conclusion: </b>The transplantation of ucc leads to the repair of the severed sciatic nerve. Also, the PLGA acts as a scaffolding to place cord stem cells and<i> cil</i> by reducing inflammation caused by surgery.</span><br /> <br /> &nbsp;</span><br /> <br /> &nbsp;</span></span></p> Cichorium intybus L.,mesenchymal stem cells,poly lactic-co-glycolic acid scaffold,sciatic nerve,neural repair 0 0 http://ppj.phypha.ir/browse.php?a_code=A-10-2633-1&slc_lang=en&sid=1 amirhosein fazlali ahfazlali@ahoo.com 3200319475328460039064 3200319475328460039064 No PhD student of Cell Biology - Islamic Azad University - Science and Research Branch-Tehran, Iran Nasim Hayati-Roodbari hayati@srbiau.ac.ir 3200319475328460039065 3200319475328460039065 Yes Associate Professor, Department of Cell Biology, Faculty of Basic Sciences, Islamic Azad University,Research Branch, Tehran, Iran. Gholam Reza Kaka gh_kaka@yahoo.com 3200319475328460039066 3200319475328460039066 No Professor of Anatomy, Baqiyatallah University of Medical Sciences - Tehran, Iran KAZEM PARIVAR k.parivar@srbiau.ac.ir 3200319475328460039067 3200319475328460039067 No Professor, Department of Cellular Developmental Biology, Faculty of Basic Sciences, Islamic Azad University - Science and Research Branch - Tehran, Iran Mohammad Kamalinejad mkamalinejad@yahoo.com 3200319475328460039068 3200319475328460039068 No Expert in Pharmacognosy Department, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran