en Cell, Stem Cell and Cancer Cell, Stem Cell and Cancer Experimental research article Experimental research article <p><span style="line-height:2;"><span style="font-size:12px;"><span style="font-family:Arial;"><span style="background:white"><span style="unicode-bidi:embed"><span calibri=""><b><span new="" roman="" times=""><span style="color:#222222">Background: </span></span></b><span new="" roman="" times=""><span style="color:#222222">Despite various treatment methods, the damaged peripheral nervous system has become a severe problem in today&#39;s societies.</span></span></span></span></span><br /> <span style="background:white"><span style="unicode-bidi:embed"><span calibri=""><b><span new="" roman="" times=""><span style="color:#222222">Objectives:</span></span></b><span new="" roman="" times=""><span style="color:#222222"> This study aimed to evaluate the effects of cultured bone marrow stromal cells (BMSCs) on the chitosan-gelatin membrane and olive fruit extract in Wistar rat cutaneous sciatic nerve repair.</span></span></span></span></span><br /> <span style="background:white"><span style="unicode-bidi:embed"><span calibri=""><b><span new="" roman="" times=""><span style="color:#222222">Methods: </span></span></b><span new="" roman="" times=""><span style="color:#222222">The chitosan-gelatin membrane was prepared after extraction of olive and extraction of BMSCs and cultivation in special pellets. Wistar rats were divided to 9 groups (n=5): </span>healthy control, sciatic nerve injury(sni), sni<span style="color:#222222"> treated with </span>bone marrow stromal stem cells, sni<span style="color:#222222"> treated with</span> olive fruit extract, sni<span style="color:#222222"> treated with </span>olive fruit extract+bone marrow stromal stem cells, sni<span style="color:#222222"> treated with</span> olive fruit extract+chitosan-gelatin, sni<span style="color:#222222"> treated with </span>chitosan-gelatin+ bone marrow stromal stem cells, and sni<span style="color:#222222"> treated with </span>olive fruit extract+chitosan-gelatin+ bone marrow stromal stem cells<span style="color:#222222">. The treatment period was 8 weeks, then the evaluation of </span>sni<span style="color:#222222"> repair included electrophysiological, sciatic functional index, tissue sampling and immunocytochemistry, and histopathological examination.</span></span></span></span></span><br /> <span style="background:white"><span style="unicode-bidi:embed"><span calibri=""><b><span new="" roman="" times=""><span style="color:#222222">Results: </span></span></b><span new="" roman="" times=""><span style="color:#222222">In the group treated with </span>olive fruit extract +chitosan-gelatin+ bone marrow stromal stem cells<span style="color:#222222">, sciatic nerve repair was observed with an increase in nerve conduction velocity (m/s), amplitude muscle action potential (mv), number of (vessels and fiber), most significant area of vessels, myeline, axon, and fiber (1000 micro m2) and a decrease in statistic functional index (0 to -50) (P<0.0001).</span></span></span></span></span><br /> <span style="background:white"><span style="unicode-bidi:embed"><span calibri=""><b><span new="" roman="" times=""><span style="color:#222222">Conclusion: </span></span></b><span new="" roman="" times=""><span style="color:#222222">The combination of olive extract, chitosan-gelatin, and bone marrow stromal stem cells (BMSCs) demonstrated a significant reparative effect on sciatic nerve injury and could serve as a promising approach for peripheral nerve repair.</span></span></span></span></span><br /> <span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><span dir="LTR"><span style="line-height:115%"></span></span></span></span></span><br /> &nbsp;</span></span></span></p> Peripheral nervous system,Sciatic nerve,Chitosan-gelatin scaffold,Olive fruit extract,Bone marrow stromal stem cells 0 0 http://ppj.phypha.ir/browse.php?a_code=A-10-2636-1&slc_lang=en&sid=1 mostafa moazami mostafamoazami1986@gmail.com 3200319475328460039069 3200319475328460039069 No PhD student of Cell Biology - Islamic Azad University - Science and Research Branch-Tehran, Iran Nasim Hayati-Roodbari hayati@srbiau.ac.ir 3200319475328460039070 3200319475328460039070 Yes Department of Cell Biology, Faculty of Basic Sciences, Islamic Azad University,Research Branch, Tehran, Iran. KAZEM PARIVAR k.parivar@srbiau.ac.ir 3200319475328460039071 3200319475328460039071 No Professor, Department of Cellular Developmental Biology, Faculty of Basic Sciences, Islamic Azad University - Science and Research Branch - Tehran, Iran Mohammad Kamalinejad mkamalinejad@yahoo.com 3200319475328460039072 3200319475328460039072 No Expert in Pharmacognosy Department, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran gholamreza kaka gh-kaka@yahoo.com 3200319475328460039073 3200319475328460039073 No Gholamreza KakaProfessor of Anatomy andNeuroscience Research Center, Baqiyatallah University of Medical Sciences,