Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1387
2
1
gregorian
2008
5
1
12
1
online
1
fulltext
en
Alterations of noradrenalin and one of its metabolites in the Locus Coeroleus nucleus in formalin induced pain in anesthetized rat
Pain and addiction
Neurophysiology/Pharmacology
Experimental research article
Experimental research article
Introduction: Pain as a complex process in central nervous system (CNS) has been studied by many researchers.
Pain is controlled by several CNS pathways, one of the most important of which, is the descending noradrenergic
system. This system begins from locus coeruleus (LC) nucleus in pons and ends in the spinal cord. In this research, the
effect of pain induced by formalin was studied.
Methods: Male Sprague-Dawley rats weighing 280-320 g were categorized into two groups of control (injection of
50 μl normal saline) and test (injection of 50 μl 2.5% formalin). Rats were anesthetized by pentobarbital sodium (50
mg/kg i.p.). Microdialysis probes were inserted 24 hrs before the test was done. Rats were anesthetized by urethane and
formalin test for induction of chemical and tonic pain was performed on the hind paw of the animals. Micro dialysis
samples were taken in 15 minutes intervals and noradrenaline (NA) and its metabolite, 3-methoxy 4-hydroxy
phenylglycol (MHPG), were measured by HPLC-ECD.
Results: The NA and MHPG concentration in the first and second phases of formalin test did not change
significantly in neither test nor control groups.
Conclusion: LC has no role in perception of pain induced by formalin test during anesthesia.
Pain, Formalin test, locus coeruleus, Microdialysis, Noradrenaline.
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Javad
Sajedianfard
Sajedianfard
320031947532846004052
320031947532846004052
Yes
Faramarz
Azarang
320031947532846004053
320031947532846004053
No
Elahe
Solimannejad
320031947532846004054
320031947532846004054
No