Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1387
5
1
gregorian
2008
8
1
12
2
online
1
fulltext
en
Study of the effects of intra-nucleus accumbens shell injections of alcohlic extract of Crocus sativus on the acquisition and expression of morphine-induced conditioned place preference in rats
Pain and addiction
Neurophysiology/Pharmacology
Experimental research article
Experimental research article
Background: Previous studies have confirm the effects of water extract of Crocus sativus on the euphoric and behavioral properties of morphine in mice.
Objective: In the present study, the effects of intra-accumbal administration of alcohol extract of Crocus sativus stigma on the acquisition and expression of morphine-induced conditioned place preference (CPP) in male Wistar rats (250-300 g) were investigated.
Material and Methods: This experimental study was conducted on the 78 male rats that were divided in 18 groups (n=6/group). In a pilot study, different doses of morphine (0.5, 1, 2.5, 5, 7.5 and 10 mg/kg) were injected to the animals for evaluation of the drug's ability to induction of place preference. In the second phase of the experiments, the extract of the C. sativus (1, 5 and 10 µg/rat), was administered into the nucleus accumbens shell during or after induction of morphine CPP. Then, CPP were tested in the animals. One-way Analysis of Variance (ANOVA) was proformed for statistical procedure.
Results: Administration of morphine (0.5, 1, 5, 7.5 and 10 mg/kg), indcreased the time spend in the compartment paired with morphine (i.e. conditioned place preference-CPP). The increament was significant for the dose 10 mg/kg of morphine. Injection of the same doses of the extract (1, 5 and 10 µg/rat) 5 min before morphine (10 mg/kg) administration, caused a decrease in the time spent in drug-paired side in doses 5 and 10 µg/rat of the extract. In addition, injection of the plant extract (1, 5 and 10 µg/rat) in to the shell part of nucleus accumbens in the test day to the animals in which reveived morphine (10 mg/kg) in the conditioning days decreased the expression of morphine CPP in the animals which was statisticaly significant for doses 5 and 10 µg/rat of the extract.
Conclusion: It could be concluded that intra-accumbens shell compartment njection of the alcoholic extract of C sativus can inhibit the acquisition and expression of morphine-induced CPP and shift it to the aversive state in rats.
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Morphine, Conditioned place preference, Rats, Saffron Extract
121
128
http://ppj.phypha.ir/browse.php?a_code=A-10-74-46&slc_lang=en&sid=1
Narges-al-sadat
Mojabi
320031947532846004002
320031947532846004002
No
Azad Islamic University
Akram
Eidi
320031947532846004003
320031947532846004003
No
Azad Islamic University
Mohammad
Kamalinejad
320031947532846004004
320031947532846004004
No
Shaheed Beheshti University of Medical Sciences
Ali
Khoshbaten
320031947532846004005
320031947532846004005
No
Baqyiatallah (a.s.) University of medical Sciences
Ali
Noroozzadeh
320031947532846004006
320031947532846004006
No
Baqyiatallah (a.s.) University of medical Sciences
Hedayat
Sahraei
h.sahraei@bmsu.ac.ir
320031947532846004007
320031947532846004007
Yes
Baqyiatallah (a.s.) University of medical Sciences
Farial
Khamseh
320031947532846004008
320031947532846004008
No
Baqyiatallah (a.s.) University of medical Sciences
Farzaneh
Zighimat
320031947532846004009
320031947532846004009
No
Baqyiatallah (a.s.) University of medical Sciences