en Pain and addiction Neurophysiology/Pharmacology Experimental research article Experimental research article Introduction: Although formalin-induced activity in primary afferent fibers and spinal dorsal ‎horn is well described, the midbrain neural basis underlying each phase of behavior in ‎formalin test has not been clarified. The present study was designed to investigate the nucleus ‎cuneiformis (CnF)‎‏ ‏neuronal responses during two phases after subcutaneous injection of ‎formalin into the hind paw of rat.‎ Materials & Methods: In this study, seventy six male NMRI adult rats, weighing 230-320 g ‎were used. Control group (n=24), which was tested merely for determining spontaneous firing ‎rate of CnF neurons. Saline group (n=15) which received saline (50µl s.c.) instead of ‎formalin into the plantar surface of hind paw after 15 min baseline recording. Formalin group ‎that formalin-induced neural activity of 37 cells simultaneously recorded from the CnF during ‎first phase (0-5 min) and second phase (15-60 min) of formalin test in 5-min intervals, using ‎an extracellular single unit recording technique.‎ Results: The‏ ‏baseline firing rate of neurons in the CnF varied between 1.2 and 39.2 spikes/sec ‎and the average frequency of spontaneous activity over 1 h was 11.8 ± 1.1 spikes/sec. There ‎were three neural clusters after formalin injection. Neurons in cluster 1 (46%) exhibited ‎severe, transient excitatory response in the first (acute) phase while neurons in cluster 2 (35%) ‎exhibited tonic but long-lasting excitatory response in the second (chronic) phase. Cluster 3, a ‎small portion of neurons (about one fifth) which failed to show any evident responses to ‎formalin test. ‎ Conclusion: Our findings suggest that alteration of neural activity and pattern in the ‎spontaneous background of CnF neurons can be mediated a role in the transmission of ‎nociceptive information induced by the peripheral injection of formalin and can be discussed ‎in light of the role of these neurons in nociceptive information processing following ‎peripheral stimuli.‎ Nucleus cuneiformis, Formalin test, Firing rate, Single unit recording, Rat 101 108 http://ppj.phypha.ir/browse.php?a_code=A-10-5-1&slc_lang=en&sid=1 Abbas Haghparast Haghparast@yahoo.com 320031947532846004016 320031947532846004016 Yes Shahid Beheshti University, M.C. Amir-Mohammad Alizadeh Amir.m.alizade@gmail.com 320031947532846004017 320031947532846004017 No Shahid Beheshti University, M.C. Fereshteh Motamedi Motamedi@ams.ac.ir 320031947532846004018 320031947532846004018 No Shahid Beheshti University, M.C.