en A2C8 and MC2B8 Monoclonal Antibodies Against Human Plasminogen inhibit Fibrinolytic System Experimental research article Experimental research article <b><font face="TimesNewRomanPS-BoldMT" size="2"><p align="left">A Maleki</p></font><font face="TimesNewRomanPS-BoldMT" size="1">1</font><font face="TimesNewRomanPS-BoldMT" size="2">, M Mirshahi</font><font face="TimesNewRomanPS-BoldMT" size="1">2</font><font face="TimesNewRomanPS-BoldMT" size="2">, AA Pourfathollah</font><font face="TimesNewRomanPS-BoldMT" size="1">1 </font><font face="TimesNewRomanPS-BoldMT" size="2">and K Mansouri</font><font face="TimesNewRomanPS-BoldMT" size="1">1</font><i><font face="TimesNewRomanPS-BoldItalicMT" size="2"><p align="left">1. Dep. Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.</p><p align="left">2. Dep. Biochemistry, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran.</p></font><font face="TimesNewRomanPS-BoldMT" size="3"><p align="left">Abstract:</p></font><font face="TimesNewRomanPSMT" size="3"><p align="left">In the present study the effects of two antiplasminogen monoclonal antibodies A2C8 and MC2B8 on the activation</p><p align="left">of the fibrinolytic system was studied.</p><p align="left">For this purpose, after separate steps like the culture of antibody producing hybridoma cells, their injection to</p><p align="left">mice, and purification of antibodies from ascites fluid, the effects of these antibodies was studied with various</p><p align="left">methods. Primary observation with pooled plasma showed that these antibodies in the presence of plasminogen</p><p align="left">activators (t-PA, u-PA and SK) can inhibit the activation of fibrinolytic system. According the results obtained</p><p align="left">from D-dimer assay, the inhibition effects of these antibodies is dose dependent. The other test that performed</p><p align="left">with S-2251 synthetic substrate showed that the activation of plasminogen in the presence of Urokinase and in the</p><p align="left">result, deterioration of this substrate was decreased in the presence of A2C8 and MC2B8 antibodies.</p><p align="left">In the present study we evaluated the effects and mechanism of two antiplasminogen monoclonal antibodies A2C8</p><p align="left">and MC2B8 on the activation of the fibrinolytic system in the presence of plasminogen activators.</p></font><b><font face="TimesNewRomanPS-BoldMT" size="3"><p align="left">Keywords: </p></font><font face="TimesNewRomanPSMT" size="3">Fibrinolytic system, Plasminogen, Monoclonal antibody, Plasminogen activators.</font></b></i></b> 203 209 http://ppj.phypha.ir/browse.php?a_code=A-10-57-1&slc_lang=en&sid=1