en Nervous system (others) Others Experimental research article Experimental research article Introduction: Demyelination is one of the main causes of neurological disability. It is the end product of numerous pathological processes, multiple sclerosis (MS) being the most common cause. More than 70% of the MS patients suffer from optic disturbances. This disease commonly affects the optic pathway, particularly the optic nerves and chiasm. Several attempts have been made to produce a suitable model of demyelination in optic apparatus up to now. Methods: Local demyelination model was generated using direct injection of lysolecithin (LPC) into the optic chiasm of C57/BLJ6 mice without any undesirable distributions of gliotoxin into other brain structures. Histological and electrophysiological assessments of the processes of demyelination and remyelination in the animal model were done with specific myelin staining and visual evoked potential (VEP) recordings. Results: In this study, both electrophysiological and histological results demonstrated that maximum level of demyelination was observed on day 7 post lesion and an incomplete yet significant remyelination took place on day 14 post lesion. Conclusion: Results showed a relatively rapid endogenous myelin repair in mice optic chiasm. Furthermore, this report might offer a new tool to address possible involvement of new origins of myelin-forming cells and subsequently their manipulation to promote myelin repair in the adult central nervous system. Lysolecithin, Demyelination, Myelin repair, Optic chiasm, Visual evoked potential (VEP), Mouse 324 336 http://ppj.phypha.ir/browse.php?a_code=A-10-448-1&slc_lang=en&sid=1 Fereshteh Pourabdolhossein pourabdolhossein@gmail.com 320031947532846008243 320031947532846008243 No physiology department, Tarbiat Modares university Sabah Mozafari sabahmozafari@gmail.com 320031947532846008244 320031947532846008244 No physiology department, Tarbiat Modares university Mohammad Javan mjavan@modares.ac.ir 320031947532846008245 320031947532846008245 Yes physiology department, Tarbiat Modares university Sied Javad Mirnajafizadeh MIRNAJAF@modares.ac.ir 320031947532846008246 320031947532846008246 No physiology department, Tarbiat Modares university Abolhassan Ahmadiani 320031947532846008247 320031947532846008247 No Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran