Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1389
10
1
gregorian
2011
1
1
14
4
online
1
fulltext
en
Comparison between the effects of L-Carnitine and Acetyl-L-Carnitine on myocardial infarction size in ischemic heart
Pharmacokinetics/Dynamics
Pharmacokinetics/Dynamics
Experimental research article
Experimental research article
Introduction: In the present study, potential differences between the effects of L-carnitine (LC) and acetyl-Lcarnitine
(ALC) on ischemia/reperfusion (I/R)-induced myocardial infarction size were investigated.
Methods: Male Wistar rats were randomly divided into five groups and then anesthetized by sodium pentobarbital.
Hearts of the animals were removed and quickly mounted on a Langendorff apparatus and perfused under constant
pressure by a modified Krebs-Henseleit (K/H) solution. The hearts were perfused during 30 min regional ischemia
followed by 120 min reperfusion by normal K/H solution (as control) or enriched solution with 1.5 and 3 mM LC
(groups 2 and 3) or ALC (groups 4 and 5). At the end of the reperfusion cycle, 0.25 % evans blue solution was infused
to stain the non-ischemic area, then the hearts were cut into slices and incubated by triphenyltetrazolium chloride
solution and fixed by formalin. The infarction size was determined by using a computerized planimetry package.
Results: The infarct size after perfusion of isolated hearts with 1.5 (26±4.5%) and 3 mM LC (20±4 %) showed a
significant reduction compared to the control value (45.6±3.4 %, P<0.01 and P<0.001, respectively). The same
concentrations of ALC significantly lowered myocardial infarction size to 23.8±4 % (P<0.01) and 15.8±2.9 %
(P<0.001), respectively. When the effects of similar concentrations of LC and ALC on the infarction size were
compared, there was no statistically significant difference (p>0.05). Among the potential protective mechanisms of the
agents, increase of glucose oxidation, reduction of lactate production, toxic fatty acid metabolites and removing free
radicals from the myocytes seem to be more relevant.
Conclusion: The results of this study demonstrated the protective effects of LC and ALC against I/R injuries by
reduction of infarct size in isolated rat hearts without any important difference between the agents.
L-carnitine, Acetyl-L-carnitine, Infarction size, Isolated heart, Rat
380
388
http://ppj.phypha.ir/browse.php?a_code=A-10-168-2&slc_lang=en&sid=1
Moslem
Najafi
najafimoslem@yahoo.com
320031947532846008232
320031947532846008232
Yes
School of Pharmacy
Saba
Ghaffary
saba_gh_64@yahoo.com
320031947532846008233
320031947532846008233
No
School of Pharmacy
Elnaz
Shaseb
elnaz_shaseb_64@yahoo.com
320031947532846008234
320031947532846008234
No
School of Pharmacy
Tahereh
Eteraf Oskouei
eteraf_t@yahoo.com
320031947532846008235
320031947532846008235
No
School of Medicine