Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1390
1
1
gregorian
2011
4
1
15
1
online
1
fulltext
en
Electrophysiological characteristics of hippocampal CA1 neurons after spreading depression-triggered epileptic activity in brain slices
Nervous system (others)
Others
Experimental research article
Experimental research article
Introduction: A close link between spreading depression (SD) and several neurological diseases such as epilepsy
could be demonstrated in many experimental studies. Epilepsy is among the most common brain disorders. Despite a
large number of investigations, its mechanisms have not been yet well elucidated. Hippocampus is one of the important
structures involved in seizures. The aim of this study is to get an insight into the patho-physiological processes induced
by SD that lead to the generation of epileptiform field potentials.
Methods: The horizontal amygdala-hippocampus-neocortex slices of rat brain in which SD was induced by KCl
application in each brain structure were used. Following GABAA receptor antagonist bicuculline superfusion (1.25
μmol/L, for 45 min), SD induced epileptic activity in all tested slices was monitored.
Results: The induction of SD in the hippocampus resulted in interictal and ictal epileptiform field potentials and
intracellular paroxysmal depolarization shifts (PDS). After SD, RMP slightly depolarized and the threshold of AP
decreased, while the frequency of AP significantly increased. Amplitude of depolarization and also amplitude of
discharges were also significantly increased. ISI significantly decreased and the most of neurons shifted from FA to SA
indicating an enhanced excitability.
Conclusion: SD may cause pathological changes in brain structures such as increased excitation and/or decreased
inhibition. Propagation of SD over epileptogenic areas may trigger seizure attacks in some patients and our findings
provide evidence on the role of SD in temporal lobe epilepsy.
Key words: Epilepsy, Horizontal slices, Spreading depression, Ictal burst activity, Bicuculline
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http://ppj.phypha.ir/browse.php?a_code=A-10-21-132&slc_lang=en&sid=1
Parviz
Shahabi
320031947532846008856
320031947532846008856
No
Ali
Gorji
gorjial@uni-uenster.de
320031947532846008857
320031947532846008857
Yes
Yaghoub
Fathollahi
fatolahi@modares.ac.ir
320031947532846008858
320031947532846008858
No
Mahyar
Janahmadi
320031947532846008859
320031947532846008859
No
Javad
Mirnajafi-zade
320031947532846008860
320031947532846008860
No
Jalil
Arabkheradmand
320031947532846008861
320031947532846008861
No
Marius
Alexander Eickhoff
320031947532846008862
320031947532846008862
No